Hi: 
I hoping someone out there may have a solution to a problem we have in our
lab.  The jist of it is that the edges of the eye corneas on our slides have
been lifting and folding back.  It doesn't matter if we use adhesive slides
or not.  The problem mainly occurs on rat eyes and not as much on the larger
species.  Our procedure is as follows:  We want to prevent retinal
detachment so the eyes are fixed in a solution that has acetic acid, alcohol
and conc. formalin.  Recently, we tried Davidson's fixative which differs by
using 10%NBF instead of conc. formalin.  Both have the problem of lifting
corneas.  We don't have the problem when just fixing in 10% NBF, but then we
have retinal detachment occuring. If we go back to using 10%NBF can anyone
suggest ways of preventing detachment from happening?  Any help sent our way
is greatly appreciated.
Thanks.....
Judi Ford
judi.ford@roche.com