saccomano's fixative

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From:Pat Stockton <stockton@niehs.nih.gov> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Would love to hear from anyone who has used/heard of Saccomano's (sp?)
fixative.
Big thanks!
pat

>X-Sender: foley1@pop.niehs.nih.gov
>Mime-Version: 1.0
>Date: Mon, 19 Oct 1998 09:37:21 -0400
>To: STOCKTON@niehs.nih.gov
>From: Julie Foley <foley1@niehs.nih.gov>
>Subject: saccomano's fixative
>
>Pat,
>Can you do a posting on the histo site and see if you get any info on this
>one for Rob?
>Thanks
>Julie
>
>
>
>>X-Sender: slebos@drucker.niehs.nih.gov (Unverified)
>>Date: Sun, 18 Oct 1998 23:54:25 -0400
>>To: Julie Foley <foley1@niehs.nih.gov>
>>From: Robbert Slebos <slebos@niehs.nih.gov>
>>Subject: saccomano's fixative
>>Cc: slebos@niehs.nih.gov
>>Mime-Version: 1.0
>>
>>Julie,
>>
>>Pardon my ignorance here, but I've looked and not found any decent recipe
>>for what's called Saccomano's fixative. My main interest is in the presence
>>of any cross-linkers that could mutilate our precious DNA <g>. It's for
>>bronchial washings, so not too many enzymes or other gooey stuff expected.
>>I was thinking of simply putting it in 10-20% ethanol.
>>
>>Thanks,
>>Rob.
>>
>>----------------------------------------------------------------------
>>Robbert J.C. Slebos, Ph.D.
>>Visiting Scientist
>>National Institute of Environmental Health Sciences
>>Laboratory of Molecular Carcinogenesis
>>Maildrop C2-01
>>P.O.Box 12233
>>Research Triangle Park, NC 27709
>>
>>Telephone: 919-541-2694
>>Facsimile: 919-541-2511
>>E-mail: slebos@niehs.nih.gov
>>----------------------------------------------------------------------
>>
>

Patricia Stockton, HT
Stockton@niehs.nih.gov




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