Hello Cindy;
We have been running FactorVIII for the last few years and use Protease XIV
@ 0.5
mg/ml in PBS with good results.  Our antibody is purchased from Dako.  I
would try
your usual protease method first as I suspect the stain is fairly robust,
most of the
Pepsin digestion pretreatments I have read about in the past also work just
fine
using other enzymatic protocols.

regards

BJC - Brian		PS -  I haven't forgotten about the Rota....


On Thu, 29 Oct 1998 09:42:10 +0600 Cindy Chard-Bergstrom wrote:

> From: Cindy Chard-Bergstrom <CHARD_B@vet.ksu.edu>
> Date: Thu, 29 Oct 1998 09:42:10 +0600
> Subject: Pepsin
> To: HistoNet@Pathology.swmed.edu
>
> Hello Everyone,
>
>     Has anyone out there had any experience using Pepsin as
> pretreatment for IHC's. If so what is the concentration that you have
> used. I am using factor VIII antibody and pepsin pretreatment is
> recommended. I have pepsin on had but it is not from the company that
> makes the primary antibody. Any advice is welcome.
>
> Cindy Chard-Bergstrom
> chard_b@vet.ksu.edu
>