We soak the block in a covered container of amm Hydrox.  Some have used it
in the hood as you suggested.  We rinse the block in water thoroughly
before we continue sectioning and have had to do no further treatment.



At 11:50 AM 10/22/98 -0700, P. Emry wrote:
>Hi Rebecca,
>
>Thanks for the info.  I will ask you as I did others, how do you work with
>this and keep your sinuses intact?  When I use am. hydroxide for other
>things I use it under the hood and still get some burning in my eyes and
>sinuses.  Also, do you have to wash the slide before staining or somehow
>remove the am. hydx and can you immuno. staining the slides treated this
>way?
>Thanks,
>Trisha
>U of WA, Seattle
>
>
>
>On Thu, 22 Oct 1998, Rebecca S Smith wrote:
>
>> We do the soaking at the block stage.  Turn the block face up and put drops
>> on the areas of cartilage for 5-10 minutes or so.  Try it, if it doesn't
>> make things behave better, give it a little longer.  The bone areas do not
>> seem to be affected in any way, other than how it would react to any
>> moisture soaking.  After we cut the section successfully, we drain as
>> briefly as possible.  As soon as the water is drained from beneath the
>> section we put the slide on the hot plate.  If you wait too long, you will
>> see the cartilage curling up in front of your very eyes.  NOT GOOD!
>> Anyway, I hope some of this is helpful to someone.
>>
>>
>>
>> At 04:59 PM 10/21/98 -0700, P. Emry wrote:
>> >Hi Rebecca,
>> >
>> >I have not heard of the am. hydroxide method.  What does this do the the
>> >bone part of the specimen?  How long do you leave it in it?
>> >
>> >Thanks,
>> >Trisha
>> >U of Washington
>> >
>> >
>> >
>> >On Wed, 21 Oct 1998, Rebecca S Smith wrote:
>> >
>> >> We find that soaking cartilage areas of the block with 5% ammonium
>> >> hydroxide does the trick!
>> >>
>> >>
>> >>
>> >> At 08:53 AM 10/21/98 +0200, Pierre Mainil wrote:
>> >> >Hello histonetters,
>> >> >
>> >> >Another question to cartilage lovers.
>> >> >What is the best decalcification system.
>> >> >We observe important variability with Saf O staining although using
>> >> >cetylpyrimidium.
>> >> >Has some one ever used EDTA with etylpyrimidium  during the
>> >> >decalcfication process.
>> >> >Advice and help appreciated.
>> >> >
>> >> >
>> >> >Pierre Bern
>> >> >
>> >> >
>> >>
>> >>
>> >>
>> >
>> >
>>
>>
>
>