Kathy,

I assume that you are refering to 1mM EDTA pH8.0, that is recommended by
some suppliers for some of their antibodies.
We also use EDTA at pH6.0 for other antibodies.

Regards

Bryan

>----------
>From: 	Kathy Oprea[SMTP:koprea@calc.vet.uga.edu]
>Sent: 	October 19, 1998 3:21 PM
>To: 	histonet@pathology.swmed.edu
>Subject: 	what is "standard" pH for EDTA?
>
>This question has come up in our department and I did not have the
>answer- sooooo, guess who I thought of? Histonet!!  :-)  Does anyone
>out there know the standard pH for EDTA used for antigen retrieval?
>My experience is strictly with citrate buffer. Thank you all in
>advance. I will post answers I receive.
>
>Kathy Oprea
>************ Thank you ************
>* Kathy Oprea                     *
>* University of Georgia           *
>* College of Veterinary Medicine  *
>* Athens, GA 30602-7388           *
>* KOPREA@calc.vet.uga.edu         *
>The worker who's never said "Thank
>God it's Friday," probably never
>really worked.
>************************************
>
>