Re: 4% paraformaldehyde
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Wed, 14 Oct 1998 NTT@shcc.org wrote:
> Does anyone have a recipe for 4% paraformaldehyde that they would be
>willing to
> share? Hopefully someone will be able to get back to me by the end of
>today.
Add 40 g paraformaldehyde and 6.5 g dibasic sodium
phosphate (Na2HPO4) to about 750 ml of water. Heat
to 60C, with magnetic stirring. The slution should
become transparent when it reaches 60C. Add 4.0 g
of monobasic sodium phosphate (NaH2PO4.H2O) and
let it cool down. Add water to one litre.
Check the pH (7.2-7.4), adjusting if necessary.
Note that this solution and others like it are
NOT "4% paraformaldehyde." They don't contain
any paraformaldehyde at all, only formaldehyde
(actually methylene hydrate) formed by
depolymerization.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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