Gelatin in Immuno water baths... YES or NO ???

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From:sjrugby@juno.com (SANDY J JULSING) (by way of histonet)
To:histonet <histonet@magicnet.net>
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Histo-experts,
	There are some in the lab where I work that don't feel it is
necessary  to use a clean (sta-on free) water bath for
immunohistochemistry.  The times that they "forgot" , there was no
noticble background problems on the resulting immuno-stained slides.
	I was always told to use a clean water bath with no additives. If
there is apparently not a problem with background, is there any other
reason that we shouldn't use our routine water bath that contains
"sta-on"?
			Many thanks, Sandosis

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