While cutting myh teeth in research many years ago, I performed rat vaginal smears and did indeed use the PAP stain. It worked very nicely.=0A________________________________From: "Della Speranza, Vinnie" To: Helen E Johnson ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, October 29, 2008 5:07:50 PMSubject: RE: [Histonet] rat vaginal smearsYou didn't indicate what you will be evaluating but your request is reminiscent of the work George Papanicolau did with guinea pigs. Have you considered the pap stain? Here is a link that will provide a lot of information if this stain is of interesthttp://www.statlab.com/Protocol/CytologyStaining.htmI like to think of the pap stain as a 'suped up' H&EVinnie Della SperanzaManager for Anatomic Pathology ServicesMedical University of South Carolina165 Ashley Avenue Suite 309=0ACharleston, South Carolina 29425Tel: (843) 792-6353Fax: (843) 792-8974-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E JohnsonSent: Wednesday, October 29, 2008 1:45 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] rat vaginal smears=0ADoes anyone have a protocol for staining rat vaginal smears with H&E?=A0 Helen Johnson=A0 (hej01@health.state.ny.us)IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
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