Try staining by coverslipping the freshly sectioned tissue using water and a glass coverslip. Put the slide edge down on a towelette and apply the stain with a dropper to the top edge of the slide. The stain will spread under the coverslip as the water escapes into the absorptive towelette underneath. Wash the same way and move on to the next reagent.
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
Florida Hospital Winter Park
janet.bonner@FLHOSP.org
407-646-7559
________________________________
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Barone, Carol=20
Sent: Thu 11/6/2008 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ??? once again..is anyone out there?
???
Any recommendations for either of these:
1.Processing and cutting of longitudnal sections of PVFD fibers...sometimes called "hollow fibers". We are having issues keeping the sections on the slides, no matter what slides we use/ or coat?
2.Di I staining...who has a histology protocol....for Di I staining?
I know there are researchers out there somewhere...???? You know you love this wierd stuff!
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