Hi Allistar: I fix my air-dried ( the tech that cuts the frozens air dries
for 60 minutes at room temp prior to placing in a plastic box and freezing
the sections. When I get ready to stain them for IHC or regular staining I
remove the sections that I need and place them immediately in cold 100%
Acetone for 20 minutes. I then proceed with my protocol.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl@uams.edu email
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alistair
Lindsay
Sent: Thursday, October 16, 2008 3:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pig endothelium....again
Dear Histonetters,
You may recall my earlier post on rings of pig aorta. I have managed to
keep them alive quite nicely with DMEM - many thanks for the tips.
Unfortunately, I am still struggling to stain for endothelial adhesion
molecules.
I have read and received various different opinions on fixation after I have
made my cryostat sections. Should the slides be fixed immediately, or air
dried first? Acetone or formalin?
Any tips would be gratefully received.
Thank You,
Alistair
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