We use 95% ethanol without methanol added, but our absolute does contain a small amount. For us, changing the 95% made all the difference. we had switched companies, and didn't realize that most ethanols had ethanol in them. Our bxs got real crunchy. When we read the 95 ingredients more carefully, we found the culprit, and when we replaced it with the non meth. 95 the bxs were gorgeous again! Our pharmacy orders the 95% from Aaper in Shelbyville,Kentucky.
jo

>>> "Weems, Joyce"  10/07/04 08:07AM >>>
This is the very first time I recall hearing that methanol might make them crispy! What kind of alcohols do you use?

Thanks, j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joanne
Mauger
Sent: Thursday, October 07, 2004 7:48 AM
To: mbecker@pathlabinc.com; histonet@pathology.swmed.edu 
Subject: Re: [Histonet] GI bx


Michele,
Could be crush artifact from clinicians or the gross bench. We use a shorter processing program for GI bxs. Make sure your 95% alc doesn't have methanol in it. This makes bxs very crispy(dries out too much)Drove us crazy too. 
Jo

>>> "Michele Becker"  10/06/04 04:21PM >>>
Please help....................  We are having problems with blurring and
distorted nuclei on some of our GI biopsies. This has been happening on and
off for the past few months.  It is not every day and not all the GI bx on
any given day. We have ruled out staining and processing. I really think it
is something happening to the bxs before we even receive them but want to
make sure we have not left any stones unturned on our end first.  Any
suggestions?

Thank-you.

Michele



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