The dual stain works great! I've used P504 for quite
some time, and recently worked up the dual stain.
For ours, we use HMW-CK and p63 together (HRP-DAB)
with p504 (AP-Fast Red). 
 
Two important things about working with p504:
    1) make sure your control has prostate CA, otherwise
you may not get staining.
    2) make sure your slides are not old -- you'll get best
results from slides under 1 month old.
 
Enjoy!
Karen Dresser
Research Histotechnician
UMass Medical School 

-----Original Message-----
From: Drew Sally A. [mailto:sa.drew@hosp.wisc.edu]
Sent: Friday, October 31, 2003 11:42 AM
To: Histonet
Subject: RE: [Histonet] AMACR


I recently worked up BioCare Medical's antibody P504S(AMACR).  
Our director anticipates it being ordered as a dual stain w/
high molecular weight cytokeratin.  We're still working
on the dual stain.  Let me know if you want more particulars.

Sally Ann Drew-MT(ASCP) 
Univ. of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. VAH-DM223 
Madison, WI 53792-2472 
608-265-6596 
sa.drew@hosp.wisc.edu 

-----Original Message-----
From: Travis Troyer [mailto:ttroyer@pcllab.com]
Sent: Friday, October 31, 2003 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AMACR


Our pathologist here was interested in the following antibody, alpha-methacryl-CoA racemase.  I was wondering in anyone uses this antibody or knows of a vendor for the antibody.  
Thanks,
Travis Troyer
Peterson Clinical Lab
Manhattan, KS 


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