Dear Histonetters

I have a quick question,  I was wondering if anybody has worked with Medial 
collateral ligament of the rat in immuno-fluoresces  staining?  If you have, 
did you have to do any type of epitope exposure to the tissue?
I know companies offer pre made solutions, but have no clue if I need to use 
this solution. Considering that I am not going there paraffin embedded 
tissue, so I am not exposing the tissue to harsh conditions that would 
degrade proteins on my  tissue.

Staining so far has been questionable, so I am up for any suggestions on 
fluoresce staining on the MCL of the Rat.

thanks again
Andrew L
lab tech
University of Houston

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