Glass Pasteurs have the advantage of being quick and easy to bend but
thicker glass makes for more comfortable handling.  If using Pasteurs
remember to seal the end as this reduces liquid transfer.

Andy Shand 

-----Original Message-----
From: louise renton [mailto:louise_renton@hotmail.com]
Sent: 29 October 2002 08:03
To: asmith@mail.barry.edu; histonet@pathology.swmed.edu
Subject: RE: TISSUE TRANSFER


We used to use glass pasteur pipettes, the fine end when heated curled over 
and rounded itself automatically.

BTW Can one still get glass pasteur pipettes?

Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





>From: "Smith, Allen" 
>To: "Featherstone, Annette" 
>CC: histonet@pathology.swmed.edu
>Subject: RE: TISSUE TRANSFER
>Date: Mon, 28 Oct 2002 11:42:10 -0500
>
>A glass "hockey stick" works well for me.  Take a short (10 cm) piece of 
>6mm
>glass rod.  Heat the end in a Bunsen burner flame and press it against a
>stone counter top to bend it 90 degrees to the rest of the rod.  If the end
>is rough, fire-polish it by holding it in the flame until the edges round
>off.
>If the sections fall off the rounded end, reheat and flatten it by grabbing
>it while still red hot with pliers.  You may need to heat the pliers and
>handle them with gloves to avoid cracking the glass.
>
>Allen A. Smith, Ph.D.
>Barry University
>School of Graduate Medical Sciences
>     Podiatric Medicine and Surgery
>Miami Shores, Florida  33161-6695
>
>-----Original Message-----
>From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org]
>Sent: Monday, October 28, 2002 10:19 AM
>To: 'HISTONET@PATHOLOGY.SWMED.EDU'
>Subject: TISSUE TRANSFER
>
>
>DOES ANYONE HAVE ANY IDEAS ON THE BEST WAY TO TRANSFER 50UM SKIN SECTIONS
>FOR PGP9.5 , FROM WELL TO WELL?
>ANNETTE


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