There have been so many inquiries about this antibody
that I decided to post to everybody. 

We are using a monoclonal version of this antibody from
a private source that is not currently commercially available.
I do not know when/if it will be, when I find out, I'll let
everyone know. 

Ordering information on polyclonal p504s:

The company Zeta is selling the antibody. Please use
the contact below for ordering information.

Peiguo G. Chu
Department of Pathology 
City of Hope National Medical Center 
1500 East Duarte Road 
Duarte, California 91010
pchu58@yahoo.com

Also
>... are you using a manual method, or automated (and if so, which system
>do you have?)


We have used the polyclonal and have gotten good staining,
but the monoclonal seems to work more specifically. Our
conditions for both are:
	zinc-formalin fixation
	routine paraffin processing
	citrate pH 6.0 microwave retrieval
	automated (Dako) or manual staining using ABC-HRP, DAB

I'll be happy to share more details with anyone who may be interested.

Hope this helps!

-- Karen Dresser
Research Histotechnician
UMass Medical School


-----Original Message-----
From: Dresser, Karen [mailto:Karen.Dresser@umassmed.edu]
Sent: Thursday, October 17, 2002 8:39 AM
To: 'Monteverde, Cheryl A Ms MEDDAC-AK'; histonet@pathology.swmed.edu
Subject: RE: (AMRCR)/P504S


Our lab is working on p504 now. Yes, it is VERY specific
for prostate adenocarcinoma. There is a polyclonal and
a monoclonal version. In our hands, the monoclonal seems
superior to the polyclonal in both specificity and lower
background staining.

-- Karen Dresser
Research Histotechnician
UMass Medical School


-----Original Message-----
From: Monteverde, Cheryl A Ms MEDDAC-AK
[mailto:Cheryl.Monteverde@nw.amedd.army.mil]
Sent: Wednesday, October 16, 2002 7:00 PM
To: histonet@pathology.swmed.edu
Subject: (AMRCR)/P504S


Has anyone out there used this antibody? It is supposed to be very specific
for prostatic adenocarcinoma. My pathologist received a flyer on it from the
marketing company (ZETA CORP), and wants me to try it. I would like to find
out more about it before ordering it, since we have never done
immunostaining here.  Any help would be greatly appreciated.

Cheryl Monteverde
Dept. of Pathology
Bassett Army Hospital
Ft. Wainwright, AK