when i had a b5 fixed sample to do TRAP on i just put up with the mercury
parcipitate, it usually did not pose an interpation problem.
patsy

"DeLovino, Salvacion S." wrote:

> In our old procedure, there was a comment section that says esterase is
> inhibited to varying degrees by mercury, acid solutions, heat, and iodine.
>
> Salvacion Delovino
> > ----------
> > From:         Johnston, Kathy[SMTP:Kathy.Johnston@CLS.ab.ca]
> > Sent:         Friday, October 11, 2002 8:39 AM
> > To:   Histonet (E-mail)
> > Subject:      Chloroacetate Esterase
> >
> > We are using Sigma's Chloroacetate Esterase kit, and are doing one on a B5
> > fixed bone marrow slide.  Our problem is thus:  Both myself and the stains
> > tech recall in the recesses of our collective memories, that Na
> > thio/iodine
> > to remove the mercury pigment produces a false negative for this test.
> >
> > Is there anyone else out there that confirms or contradicts this??  We are
> > reluctant to proceed and possibly ruin a slide, as well, we do not want to
> > stain it, and leave a bunch of mercury which may impair diagnosis.
> >
> > Your help is greatly appreciated!
> >
> > Kathy Johnston
> > Tech II - Special Stains
> > Anatomic Pathology - Foothills Medical Center
> > Calgary Laboratory Services
> > Ph - 403-944-4760
> > Fax - 403-270-4093
> > kathy.johnston@cls.ab.ca
> >
> >