When the skin is taken at necropsy place it gently on a piece of lens paper.
Place lens paper upside sown in formalin. I usually trim after fixation,
skin it straight and beautiful. Another technique is to use thin cork board
and pin the tissue. My preference is not to pin.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: David Mills [mailto:dkmills@latech.edu]
Sent: Friday, October 11, 2002 9:33 AM
To: Histonet
Subject: Skin embedding protocol


I am looking for a paraffin embedding protocol for rat skin. I need to 
minimize shrinking, tissue folding and distortion. Also any 
recommendations on a paraffin wax for soft tissues (ligaments and 
fibrocartilages)?

Thanks,

Dr. David Mills
Louisiana Tech University