Hello Elizabeth,
In our lab we perform RNA isolation on a wide variety of tissues. We 
have been using a chemical called RNAlater from Ambion, an RNA 
specialist company (www.ambion.com, search for RNAlater, and you will 
find a downloadable pdf protocol). RNAlater can be used at room 
temperature, completely inactivates RNases, providing stability to RNAs 
in your tissues, and is nontoxic. The great thing is that it is 
compatible with TRIzol RNA isolation reagent (Life Technologies) which 
we use for RNA isolation. I highly recommend it.

Phillip Huff
FBN Dummerstorf
Germany

Donato, Elizabeth M wrote:

> Hi Folks,
> 
> I was wondering if anyone has an established and tested procedure for
> freezing solid tissues (in this case breast tissue) so that the RNA is
> preserved for future retrieval and analysis?  I'm pretty familiar with
> tissue freezing and storage protocols but its the RNA preservation part that
> I need more information on.  Any feedback you might be willing to provide
> would be much appreciated.  Thanks!
> 
> Liz
> 
> Elizabeth Donato
> Project Manager, Porter Lab
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Avenue North, C1-015
> Seattle, WA 98109-1024
> 
> Email: edonato@fhcrc.org
> Ph:   206-667-4501 
> Fax:  206-667-5815
> 
> 
>