Bone processing
Dear Gayle,
I am still trying to perfect my bone/tendon sample processing and
cutting etc. I am getting there thanks to your help. I just have a
few more queries. Hope it's ok.
I want to set up a vacuum infiltration system in my lab. I suppose
any vacuum dessicator will work? I would probably need a glass one
to put it in the 58 degree oven. Is a Wheaton O ring dessicator
special in any way? I have a glass dessicator in mind and this has a
silicon ring.
I will follow the times you recommended for processing as the pieces
of bone are quite large. I am going to process as follows: 70%
ethanol x2 11/2 hrs each, 90% ethanol x2 11/2 hrs each, 100%
ethanol x3 11/2hrs each, xylol x2 11/2 hrs each, histosec wax x2( at
60degrees) 11/2 hrs each. (all hopefully under vacuum) Does this
sound ok.?
When you say drain and then dry the sections flat at 40C for several
days how many days is several. ? Why does drying them flat help and
why not at 60C ? I normally drain them and then put them on their
sides in a rack in the 60C oven overnight. I use aptes coated slides
as opposed to gelatin.
I am at a lost as to which Hematoxylin to use. I usually use
Mayer's routinely but I see you do not recommend it. I used Mayer's
with a celestine blue treatment prior to staining and it was fine. It
could be darker though. Erlich's takes to long to ripen. I probably
will not be able to purchase Richard Allan Hx. 1 here. I will try
Gill II , I must find the method to make it on the internet.
I really hope you do not mind this long discussion.
Thanks so much for you help.
Sharon Marshall
<< Previous Message | Next Message >>