Dear netters,

	I've been attempting to do immunogold labelling of 4% paraformaldehyde
fixed human natural killer cells (pre- and post- embedding). We're
trying to label against perforin, but haven't had success. A monoclonal
antibody we have labels the protein in cells fixed with methanol, but we
need to preserve the ultrastructure of the cells. Is anyone using an
antibody against the protein that works well in paraformaldehyde fixed
cells? If so, would you please tell me what vendor you purchased it
from, and perhaps share your protocol? Thank you.

Edward Haller
University of South Florida
Pathology Department
Tampa, Florida(813)974-9584