yes, we had this problem with cd34 staining more than endothelial cells,
we switched to cd31 for more specific staining of just endothelial
cells, see my poster on this subject at:  www.ihcrg.org
patsy ruegg

Karen M Majahad wrote:

> I was wondereing if anyone has used the Novo castra CD34
> antibody. I recently tryed the antibody at a 1:25 dilution
> with a trypsin digestion and did achieve a strong staining
> of vessels. When I tryed the same dilution of the antibody
> 1:25 and used a Citrate solution in a steamer for antigen
> unmasking I achieved a good stain for the endothelial cells
> but it appears as though some of my human fibroblast may
> also be staining. Has anyone had a problem with CD34
> staining their  human fibroblast?
>
> kmajahad@tktx.com