Re: Nuclear Fast Red
From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
On Mon, 23 Oct 2000, Bennett, Catherine (Katie) wrote:
> Was wondering if Nuclear Fast Red can be cleared in Xylene for
> coverslipping.
Yes. It resists alcohol and xylene, but if this is to
counterstain something immunohistochemical, make sure
your principal end-product is also insoluble. The DAB
oxidation product is, but most alkaline phosphatase
end products are extracted by alcohol or xylene.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
E-mail: kiernan@uwo.ca
<< Previous Message | Next Message >>