You can certainly use -20 for short term storage (days) of muscle but if you
want to demonstrate enzyme activity after weeks or months -70 or liquid
nitrogen is better.

We have no problem storing antibodies at -20.

Andy Shand
----- Original Message -----
From: Mary L North <northstar44@juno.com>
To: <histonet@pathology.swmed.edu>
Sent: Friday, October 20, 2000 6:19 AM
Subject: Freezer storage


> I am inquiring for another tech whose -70 degree freezer has quit working
> and it is not likely to be replaced.  It was being use to store
> antibodies and also muscle biopsy tissue.  Would a freezer of -20 or even
> warmer temperature (non -frost free of course) have an adverse effect on
> the preservation of the antibodies and the muscle enzymes?
> Mary North
> St. Joseph Hospital
> Bellingham, WA
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