RE: Daily Digest
| From: | "Van Eyck, Deb" <deb.vaneyck@phci.org> |
Hi all,
Don't think my message got through yesterday. Could I please get a general
response? Is P53 used regularly and if so-
how is the grading or signout handled by your pathologists. Is a positive or
Neg ative used or a grading system as it is a gene
overexpression situation. Your input will be greatly appreciated as well as
what types of cases it is being used on and what clones are most used???
Thanks Deb Van Eyck
> -----Original Message-----
> From: HistoNet Server [SMTP:histonet@pathology.swmed.edu]
> Sent: Wednesday, October 11, 2000 12:02 AM
> To: HistoNet Server
> Subject: Daily Digest
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 01:00:32 -0500
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Lab Manners (Was Flooring in Histology ...)
>
> On Tue, 10 Oct 2000, Kathy Paton wrote:
>
> > Sorry to raise a well worn issue. Wax on the floors ...
>
> This issue was much discussed but not resolved in HistoNet exchanges
> ? about a year ago. All contributors seemed to assume that it was
> impossible to work without spilling wax on the floor, leaving it
> there, and then spreading it around the building by sole of shoe.
>
> I refrained at that time from posting such questions as: "Could you
> try not to spill wax on the floor?" and "If you spill wax on the
> floor, why don't you scrape it off and clean up?" My own lab isn't
> a busy one, but since 1962 I've been in and out of histology labs
> that work their way through huge volumes of wax and must occasionally
> spill some on the floor. I have never encountered waxy floors as
> an occupational hazard.
>
> Is it not part of universal laboratory etiquette that if you break
> or spill something you clean up the mess immediately? This tradition
> began (I think) with John Dalton, whose spectacular public
> lecture-demonstrations at the Royal Institution ended with "not a
> drop spilt upon the bench." JD was a schoolmaster and amateur chemist,
> early C19, who discovered carbon dioxide and recognized what it was,
> and proposed the theory that each element was composed of atoms,
> recognizable by their characteristic weights. JD was a great thinker
> who was also blessed with a steady hand.
>
> Back to the point. Is it or isn't it ridiculous to consider
> carelessly spilled wax a serious but unavoidable occupational
> hazard? I'll stop now, to prevent premature explosion.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 02:15:10 -0500
> From: ALLISON@CARDIFF.AC.UK
> Subject: Re: Pyrex coplin jars
>
> You be careful about your bottom falling out!
> Russ
> Oh dear, as I pressed "Send" I saw Cc: histonet! Whoops
>
>
> Russ Allison,
> Dental School
> Cardiff
> Wales
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 02:15:24 -0500
> From: Kenneth Wester <kenneth.wester@genpat.uu.se>
> Subject: Antibody to rat-EGFR?
>
> Hi Histonet members.
>
> Is there anyone who have experience from immunohistochemical stainings of
> epidermal growth factor receptor (EGFR) in formalin-fixed,
> paraffin-embedded rat tissue? In my initial tests, I have used a rabbit
> polyclonal anti-EGFR from Santa Cruz Biotechnology (1005, sc03). Some
> confusing staining results make me want to confirm these with a second
> antibody. However, there seem to be difficult to find commercially
> available rat-EGFR antibodies. Greatful for any tips and advice in how to
> proceed.
>
>
> Kenneth Wester, Ph.D. phone: +46 (0)18 611 02 06
>
> Dept. of Genetics & Pathology fax: +46 (0)18 471 3432
> The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjoldsvag 20
> S-752 37 Uppsala, Sweden
>
> Kenneth Wester, Ph.D. phone: +46 (0)18 611 02 06
>
> Dept. of Genetics & Pathology fax: +46 (0)18 471 3432
> The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjoldsvag 20
> S-752 37 Uppsala, Sweden
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 02:44:17 -0500
> From: ALLISON@CARDIFF.AC.UK
> Subject: Re: Recipe of Sternheimer stain
>
> I have never heard of it and I go back further than Bob (assuming
> his web photo to be relatively recent) AND I used to do
> microbiology!
> Russ
>
>
> Russ Allison,
> Dental School
> Cardiff
> Wales
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 03:30:39 -0500
> From: Paul Klosen <klosen@neurochem.u-strasbg.fr>
> Subject: Re:
>
> At 13:01 09/10/00 -0400, Nima Farsinejad a ecrit:
> >Hello Histoneters!
> > I was wondering if anybody out there knows o any company that
> >makes Biotinylated anti-chicken IgG. I have contacted DAKO, Vector
> >Laboratories, and a couple of other companies, but no luck thus far. I
> >would appreciate any input.
>
> Try Jackson laboratories. They have antibodies against about any species,
> with lots of different labels.
>
> Paul
>
> -=-
> (o
> -)
> O
> ===============================oOo==(_)==OOo=============================
> Paul Klosen, PhD
> CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
> Universite Louis Pasteur 12, rue de l'Universite
> F-67000 Strasbourg, FRANCE
> Tel. 03.88.35.85.04 Fax. 03.88.24.04.61
> ========================klosen@neurochem.u-strasbg.fr=====================
> ====
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 05:30:56 -0500
> From: "Bartlett, Jeanine" <jqb7@cdc.gov>
> Subject: RE: Lab Manners (Was Flooring in Histology ...)
>
> John:
>
> In my experience our slippery floors in the histology lab are not due to
> wax
> being spilled and not cleaned up but more due to stray portions of
> paraffin
> ribbons and paraffin shavings that become airborne and end up on the
> floor...invisible. I would assume anyone who actually spills paraffin on
> the floor would clean it up.
>
> Sincerely,
>
> Jeanine Bartlett
> Centers for Disease Control
> Atlanta, GA
> jbartlett@cdc.gov
>
> - -----Original Message-----
> From: J. A. Kiernan [mailto:jkiernan@julian.uwo.ca]
> Sent: Tuesday, October 10, 2000 1:44 AM
> To: Kathy Paton
> Cc: 'histonet@pathology.swmed.edu'
> Subject: Lab Manners (Was Flooring in Histology ...)
>
>
> On Tue, 10 Oct 2000, Kathy Paton wrote:
>
> > Sorry to raise a well worn issue. Wax on the floors ...
>
> This issue was much discussed but not resolved in HistoNet exchanges
> ? about a year ago. All contributors seemed to assume that it was
> impossible to work without spilling wax on the floor, leaving it
> there, and then spreading it around the building by sole of shoe.
>
> I refrained at that time from posting such questions as: "Could you
> try not to spill wax on the floor?" and "If you spill wax on the
> floor, why don't you scrape it off and clean up?" My own lab isn't
> a busy one, but since 1962 I've been in and out of histology labs
> that work their way through huge volumes of wax and must occasionally
> spill some on the floor. I have never encountered waxy floors as
> an occupational hazard.
>
> Is it not part of universal laboratory etiquette that if you break
> or spill something you clean up the mess immediately? This tradition
> began (I think) with John Dalton, whose spectacular public
> lecture-demonstrations at the Royal Institution ended with "not a
> drop spilt upon the bench." JD was a schoolmaster and amateur chemist,
> early C19, who discovered carbon dioxide and recognized what it was,
> and proposed the theory that each element was composed of atoms,
> recognizable by their characteristic weights. JD was a great thinker
> who was also blessed with a steady hand.
>
> Back to the point. Is it or isn't it ridiculous to consider
> carelessly spilled wax a serious but unavoidable occupational
> hazard? I'll stop now, to prevent premature explosion.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 05:45:40 -0500
> From: Neil Hand <mpznhand@unix.ccc.nottingham.ac.uk>
> Subject: Re: HEIR on Plastic
>
> Margaret,
>
> Presumably you mean for LM studies; there are a few including McCluggage
> et
> al. (1995) Immunohistochemical staining of plastic embedded bone marrow
> trephine biopsy specimens after microwave heating. Journal of Clinical
> Pathology 48:pp840-44. This paper used Polarbed 812, which is a similar
> epoxy plastic to Araldite.
>
> Hope this is of help.
>
> Neil Hand,
> Nottingham,
> England UK
>
>
> You wrote:
>
> Has anyone out there in Histoland done any antigen retrieval on Plastic
> Sections (particularly Araldite)?
>
>
>
>
> Thanks,
> Margaret
>
> ____________________________________________________________________
> Neil Hand
> Department Histopathology, University Hospital, Nottingham NG7 2UH.
> work : Tel: (0115) 924 9924 extension 43725
> ____________________________________________________________________
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 07:04:48 -0500
> From: "Mary Bryhan" <mbryhan@northernhealth.org>
> Subject: RE: Tampers
>
> Hi, you can purchase tampers in two sizes from Alliegance... they are
> fairly
> cheap too!
>
> Mary
>
> >>> "Tarpley, John" <jtarpley@amgen.com> 10/09/00 04:56PM >>>
> I know what you're looking for, but I haven't seen them supplied with
> embedding centers for several years. You should be able to have a local
> machine shop make them to whatever size and shape you need since it should
> be an easy job for the shop to just remove a little aluminum from the top
> "sides" of a cube to make the T handle.
>
> John E. Tarpley 5-1-A
> Associate Scientist
> Amgen Inc.
> One Amgen Center Drive
> Thousand Oaks, CA 91320
>
> - -----Original Message-----
> From: N Kenneison
> [mailto:nakenneison.demon.co.uk@nakenneison.demon.co.uk]
> Sent: Monday, October 09, 2000 11:43 AM
> To: histonet@pathology.swmed.edu
> Subject: Tampers
>
>
> Hi there Histonetters
>
> If anybody can help me from Sakura or any othere supplier I am looking
> for a supplier of the small aluminium presses used when embedding
> tissue.
> Sort of T shaped when viewed side on and come in at least two sizes
> about 10 x 10 mm and 5 x 5mm.
> Any supplier would be of interest but especially in the UK - ours ave
> gone missing during a refurbishement and we wouild like to get some
> more.
> Tried the Sakura web site and catalogue but no luck - they are listed as
> beign in the original packing of the embedding centre and inthe
> handbookk but no catalogue number.
>
> Any help appreciated
> - --
> N Kenneison
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 07:31:54 -0500
> From: "Roberta Horner" <rjr6@psu.edu>
> Subject: RE: Flooring surfaces in Histology
>
> We bought Nomad mats from Fisher to place under our microtoming area.
> Stray
> ribbons that fall on it seems to stay there and twice a week our cleaning
> personnel vacuum the mats or when she is on vacation we roll it up and
> take
> it outside and give it a good shake. I have one on order for the
> embedding
> area also. The mat is 3' by 5'.
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University
>
> - -----Original Message-----
> From: Kathy Paton [mailto:Patonk@WHL.co.nz]
> Sent: Monday, October 09, 2000 11:26 PM
> To: 'histonet@pathology.swmed.edu'
> Subject: Flooring surfaces in Histology
>
> Dear All
>
> Sorry to raise a well worn issue. Wax on the floors in the microtomy
> area.
> We have moved into a new laboratory with heavy duty lino with no special
> abrasive surface.
> Within three months we had a few very nasty stingey hurtee type falls.
> With Health and Safety foremost in my
> Mind I contacted a company that supplies a special type of lino with a
> metallic inset.
> We have also established a more intensive cleaning up process.
> However the architects who are designing the remainder of the laboratory
> have assured us that this is a "process problem". And that no special
> surface should be necessary. The genius even suggested putting the
> microtomists into "little rooms" with vacuums surrounding them!!!!!!
> Has anybody some enlightening suggestions for this old problem or any
> similar experiences.
> Frank Lloyd Wright...........come in please.
> Regards
> Kathy Paton
> Team Leader
> Surgical Pathology Unit
> North Shore Hospital
> Auckland
> New Zealand
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 08:18:44 -0500
> From: "marjorie lehman" <Marjorie.Lehman@unilever.com>
> Subject: RE: Flooring surfaces in Histology
>
> I got industrial carpet runners for the embedding and microtome areas
> because
> I also had to have "nice looking" ones (we get a lot of visitors). The
> runners
> come in a limited choice of colors, can be cut to order, clean well and
> "look
> nice".
> Marjorie
>
> - -----Original Message-----
> From: Roberta Horner [SMTP:rjr6@psu.edu]
> Sent: Tuesday, October 10, 2000 8:28 AM
> To: Kathy Paton; histonet@pathology.swmed.edu
> Subject: RE: Flooring surfaces in Histology
>
> We bought Nomad mats from Fisher to place under our microtoming area.
> Stray
> ribbons that fall on it seems to stay there and twice a week our cleaning
> personnel vacuum the mats or when she is on vacation we roll it up and
> take
> it outside and give it a good shake. I have one on order for the
> embedding
> area also. The mat is 3' by 5'.
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University
>
> - -----Original Message-----
> From: Kathy Paton [mailto:Patonk@WHL.co.nz]
> Sent: Monday, October 09, 2000 11:26 PM
> To: 'histonet@pathology.swmed.edu'
> Subject: Flooring surfaces in Histology
>
> Dear All
>
> Sorry to raise a well worn issue. Wax on the floors in the microtomy
> area.
> We have moved into a new laboratory with heavy duty lino with no special
> abrasive surface.
> Within three months we had a few very nasty stingey hurtee type falls.
> With Health and Safety foremost in my
> Mind I contacted a company that supplies a special type of lino with a
> metallic inset.
> We have also established a more intensive cleaning up process.
> However the architects who are designing the remainder of the laboratory
> have assured us that this is a "process problem". And that no special
> surface should be necessary. The genius even suggested putting the
> microtomists into "little rooms" with vacuums surrounding them!!!!!!
> Has anybody some enlightening suggestions for this old problem or any
> similar experiences.
> Frank Lloyd Wright...........come in please.
> Regards
> Kathy Paton
> Team Leader
> Surgical Pathology Unit
> North Shore Hospital
> Auckland
> New Zealand
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 08:35:05 -0500
> From: Cheryl Crowder <ccrowder@mail.vetmed.lsu.edu>
> Subject: Tampers
>
> Good morning - in response to you question about tampers, I also needed
> some several years ago and could not find them. As you said, Sakura knows
>
> nothing about them. However, a seasoned Tissue-Tek representative told me
>
> she would call Tissue-Tek (through Miles) and she found the part and I was
>
> able to purchase several. You might try Miles, they still makes some
> stuff
> for the tissue line, although it's not generally known. Hope this helps.
>
> Cheryl Crowder
> Chief Technologist
> Department of Veterinary Pathology
> School of Veterinary Medicine
> Louisiana State University
> Baton Rouge, LA 70803
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 09:30:41 -0500
> From: "Bennett, Catherine (Katie)" <cbennett@lrri.org>
> Subject: RE: staining plastic-embedded sections
>
> Roger Moretz wrote:
>
> I can't speak to all of the stains mentioned, but I can say that H&E,
> Masson's, PAS and methyl green-pyronin all work on JB4/gma sections at 1
> to
> 5 microns. There are some caveats. First, the staining is much lighter
> for
> thinner sections (go fig :-)).......
>
> One reason the staining is much lighter in GMA than in paraffin is that
> the
> sections are thinner, therefore there is less tissue to pick up the
> stains.
>
> *********************************
> Catherine "Katie" Bresee Bennett
> Sr. Research Technologist
> Lovelace Respiratory Research Institute
> Albuquerque, New Mexico
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 09:31:01 -0500
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: Re: Pyrex coplin jars
>
> I learned, the hard way, to avoid extreme temperature changes. I either
> preheat solution IN coplin jar with waterbath or start it in MW (inside a
> zip lock baggie), never add cold solutions to a hot coplin jar and vice
> versa, plus wash coplin jars IN my lab. Every dishwasher we have had over
> the years was heavy handed, and chipped the begollies out of jars (they
> never paid to replace them!). For Steiner and Steiner, I preheat the
> coplin jar in a waterbath, then preheat solution in an erlenmeyer, add
> minimal ingredients to flask just before use, mix, pour into jar (with all
> temperatures the same). Predicting bottom dropping out is beyond me
> (nature of bottle glass), I often put coplin jar inside a waterfilled
> beaker inside a water bath, to contain released contents (and close lab
> door!)
>
> Takes a bit thought, but with limited budgets, a dislike for chipped
> glass,
> my jars have lasted for years. Other labs have to supply their own jars,
> I
> do not loan mine out (mean old lady syndrome!) anymore. It works!
>
>
>
> >Date: Mon, 09 Oct 2000 17:14:49 -0400
> >From: Geoff McAuliffe <mcauliff@UMDNJ.EDU>
> >Subject: Re: Pyrex coplin jars
> >To: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> >CC: histonet@pathology.swmed.edu
> >
> >Gayle Callis wrote:
> >
> >> In all my years of histowork, I never found a source of Pyrex coplin
> jars!!
> >> and would have begged, borrowed or stolen to get such a beast!!!
> >>
> >> After making other people in other labs replace these pricey little
> jars,
> >> they started to pay attention on how to handle them in heated solution
> >> situations. Had my share of the bottoms falling out but I learned!!
> >
> >So how do you do it? I have broken more than a few.
> >
> >Geoff
> >--
> >**********************************************
> >Geoff McAuliffe, Ph.D.
> >Neuroscience and Cell Biology
> >Robert Wood Johnson Medical School
> >675 Hoes Lane, Piscataway, NJ 08854
> >voice: (732)-235-4583; fax: -4029
> >mcauliff@umdnj.edu
> >**********************************************
> >
> >
> >
> >
> >
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
> 406 994-4705
> 406 994-4303
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 09:31:17 -0500
> From: "MacDonald, Jennifer" <jmacdonald@sach.org>
> Subject: RE: Flooring surfaces in Histology
>
> We had special flooring installed in the histology lab and have zero
> problems with slipping. It is by Armstrong and is called "Crosswalk".
>
> > ----------
> > From: Kathy Paton[SMTP:Patonk@WHL.co.nz]
> > Sent: Monday, October 09, 2000 10:26 PM
> > To: 'histonet@pathology.swmed.edu'
> > Subject: Flooring surfaces in Histology
> >
> > Dear All
> >
> > Sorry to raise a well worn issue. Wax on the floors in the microtomy
> > area.
> > We have moved into a new laboratory with heavy duty lino with no special
> > abrasive surface.
> > Within three months we had a few very nasty stingey hurtee type falls.
> > With Health and Safety foremost in my
> > Mind I contacted a company that supplies a special type of lino with a
> > metallic inset.
> > We have also established a more intensive cleaning up process.
> > However the architects who are designing the remainder of the laboratory
> > have assured us that this is a "process problem". And that no special
> > surface should be necessary. The genius even suggested putting the
> > microtomists into "little rooms" with vacuums surrounding them!!!!!!
> > Has anybody some enlightening suggestions for this old problem or any
> > similar experiences.
> > Frank Lloyd Wright...........come in please.
> > Regards
> > Kathy Paton
> > Team Leader
> > Surgical Pathology Unit
> > North Shore Hospital
> > Auckland
> > New Zealand
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 10:24:57 -0500
> From: Sharon E Willman <sharon.willman@bms.com>
> Subject: Controls for special stains
>
> Hi,
> I need information on what tissue would be good for lipofuchsin
> staining. Any help would be most apreciated.
>
> Thanks,
>
> Sharon
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 11:01:38 -0500
> From: "Janice Mahoney" <jmahoney@alegent.org>
> Subject: Re: Tampers
>
> Hi,
> We just ordered some from Alegiance a few months ago. They are called
> tisue
> tampers. Our rep ordered them for us so I'm sorry I don't have the cat#
>
>
> Janice Mahoney
>
> >>> N Kenneison <nakenneison.demon.co.uk@nakenneison.demon.co.uk> 10/09/00
> 01:42PM >>>
> Hi there Histonetters
>
> If anybody can help me from Sakura or any othere supplier I am looking
> for a supplier of the small aluminium presses used when embedding
> tissue.
> Sort of T shaped when viewed side on and come in at least two sizes
> about 10 x 10 mm and 5 x 5mm.
> Any supplier would be of interest but especially in the UK - ours ave
> gone missing during a refurbishement and we wouild like to get some
> more.
> Tried the Sakura web site and catalogue but no luck - they are listed as
> beign in the original packing of the embedding centre and inthe
> handbookk but no catalogue number.
>
> Any help appreciated
> - --
> N Kenneison
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 11:01:59 -0500
> From: "Klemme, Nancy" <nancy.klemme@sakuraus.com>
> Subject: Re:Tampers
>
> I am resending this because yesterday's message did not post to Histonet.
> - --------------------
>
> Dear N. Kenneison (and other interested parties)
>
> Those "Tissue Tampers" are still available in the same two sizes: the
> smaller one is approximately 12.5mm square and the larger approximately
> 18.8mm
> square. Sakura,US part numbers are #1551 for the larger and #1552 for
> the
> smaller size. Available through Allegiance, VWR and Government Scientific
> Sources.
> In the U.K they are available through Bayer Diagnostics. Identify
> them
> as
> Embedding Console or TEC accessories (sorry I do not have the part number
> that
> Bayer uses, but they will know the items once you describe them.)
>
> Bayer plc
> Bayer House
> Strawberry Hill
> Newbury
> Berkshire RG14 1JA
>
> tel: 01635 563000
> fax: 01635 563393
> e-mail: corporate.communications@bayer.co.uk
>
> I hope this is helpful.
> Kind Regards,
> Nancy
>
> Nancy Klemme, HT(ASCP)
> Customer/Product Support Mgr.
> Sakura Finetek USA, Inc.
>
> _______________Reply Separator____________________
> Subject: Tampers
> Author: "N Kenneison" <nakenneison.demon.co.uk@nakenneison.demon.co.uk>
> Date: 10/9/00 10:42 AM
>
> Hi there Histonetters
>
> If anybody can help me from Sakura or any othere supplier I am looking
> for a supplier of the small aluminium presses used when embedding
> tissue.
> Sort of T shaped when viewed side on and come in at least two sizes
> about 10 x 10 mm and 5 x 5mm.
> Any supplier would be of interest but especially in the UK - ours ave
> gone missing during a refurbishement and we wouild like to get some
> more.
> Tried the Sakura web site and catalogue but no luck - they are listed as
> beign in the original packing of the embedding centre and inthe
> handbookk but no catalogue number.
>
> Any help appreciated
> - --
> N Kenneison
>
>
> - ----------
> Received: from swvx12.swmed.edu by paris.fabrik.com
> with ESMTP (Fabrik F07.3-000)
> id SINN.17997872@paris.fabrik.com ; Mon, 9 Oct 2000 13:15:35 -0800
> Received: from 129.112.18.39 ([129.112.18.39])
> by SWVX12.SWMED.EDU (PMDF V6.0-24 #46721)
> with SMTP id <01JV4YZJ2CYA9EDGHJ@SWVX12.SWMED.EDU> for
> nancy.klemme@sakuraus.com; Mon, 09 Oct 2000 15:14:01 -0500 (CDT)
> Date: Mon, 09 Oct 2000 19:42:34 +0100
> From: N Kenneison <nakenneison.demon.co.uk@nakenneison.demon.co.uk>
> Subject: Tampers
> To: histonet@pathology.swmed.edu
> Message-id: <T1$gBGAaGh45EwMl@nakenneison.demon.co.uk>
> MIME-version: 1.0
>
> - ----------
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 11:02:16 -0500
> From: "Monteverde, Cheryl A Ms BACH-Ft Wainwright"
> <Cheryl.Monteverde@nw.amedd.army.mil>
> Subject: RE: Tampers
>
> If you are in a hurry, try the hardware store...they have hex bolts on
> various sizes. I use a 3/8" and a 5/8" for most things. and if you can buy
> them loose they are only a few pennies.
>
> cheryl
>
> - -----Original Message-----
> From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu]
> Sent: Tuesday, October 10, 2000 5:30 AM
> To: histonet@pathology.swmed.edu
> Subject: Tampers
>
>
> Good morning - in response to you question about tampers, I also needed
> some several years ago and could not find them. As you said, Sakura knows
>
> nothing about them. However, a seasoned Tissue-Tek representative told me
>
> she would call Tissue-Tek (through Miles) and she found the part and I was
>
> able to purchase several. You might try Miles, they still makes some
> stuff
> for the tissue line, although it's not generally known. Hope this helps.
>
> Cheryl Crowder
> Chief Technologist
> Department of Veterinary Pathology
> School of Veterinary Medicine
> Louisiana State University
> Baton Rouge, LA 70803
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 12:03:11 -0500
> From: Dorit Zharhary <d_Zharhary@sigma.co.il>
> Subject: RE:
>
> Sigma has a biotinylated monoclonal anti-Chicken light chains (clone
> CH-31)
> Product B3400. Maybe it will do the job as good as an anti-IgG.
>
> Dorit
>
> - -----Original Message-----
> From: Nima Farsinejad [SMTP:nfarsin@emory.edu]
> Sent: a 09 aa#214#eaao 2000 19:01
> Cc: Histonet
> Subject:
>
> Hello Histoneters!
> I was wondering if anybody out there knows o any company that
> makes Biotinylated anti-chicken IgG. I have contacted DAKO, Vector
> Laboratories, and a couple of other companies, but no luck thus far. I
> would appreciate any input.
>
> Sincerely,
>
> Nima Farsinejad
> Research Specialist
> Digestive Diseases
> Emory University
> Atlanta, Georgia
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 12:03:40 -0500
> From: Miriam Schroeder <vitalred@yahoo.com>
> Subject: How/why/when do you use a "Tamper"?
>
> Hi all. I came into histology through the "back
> door", I received excellent on-the-job training but
> never any "official" or "formal" type histology
> training as I assume one would pursue to get
> certification. (I work in a research setting, not
> clinical.)
>
> Anyway, I have one of these "tamper" devices & have
> never used it. Always wondered what it was for...
> Now that I see it is called a "tamper", I imagine it
> is for holding a piece of tissue flat onto the bottom
> of the embedding mold? (I always just use forceps...)
> If that is indeed what it is for, how do you hold the
> tamper? With forceps?
>
> Thanks in advance for helping to educate me!
>
> Miriam Schroeder
>
> __________________________________________________
> Do You Yahoo!?
> Get Yahoo! Mail - Free email you can access from anywhere!
> http://mail.yahoo.com/
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 12:55:57 -0500
> From: Jill Songer <jtsonger@vt.edu>
> Subject: Re: Controls for special stains
>
> We use liver for this stain.
>
>
>
>
>
> At 11:18 AM 10/10/2000 -0400, Sharon E Willman wrote:
> >Hi,
> >I need information on what tissue would be good for lipofuchsin
> >staining. Any help would be most apreciated.
> >
> >Thanks,
> >
> >Sharon
>
>
> **************************************************************************
> Jill Songer HT (ASCP)
> Anatomic Pathology Lab Supervisor
> Veterinary Teaching Hospital
> Virginia Tech
> Blacksburg, Virginia 24061
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 13:44:48 -0500
> From: "Klemme, Nancy" <nancy.klemme@sakuraus.com>
> Subject: Re:How/why/when do you use a "Tamper"?
>
> You're right about the "tamper" use to gently flatten a larger piece of
> tissue
> during the embedding process. The greatest aid, however, is when one
> has
> scrapings or currettings - multiple tiny pieces - which you would want to
> have
> in the same plane of the paraffin block. This device would facilitate
> that
> specimen placement. Without a device such as this, the pieces of tissue
> are
> likely to be more sporadically positioned as the paraffin solidifies.
> Since
> all
> of the curettage is submitted for microscopic examination, we would want
> to
> provide the pathologist with as much of the specimen as possible for
> diagnosis.
>
> Kind regards,
> Nancy
>
> Nancy Klemme, HT(ASCP)
> Customer/Product Support Mgr.
> Sakura Finetek USA, Inc.
>
> ____________________Reply Separator____________________
> Subject: How/why/when do you use a "Tamper"?
> Author: "Miriam Schroeder" <vitalred@yahoo.com>
> Date: 10/10/00 8:53 AM
>
> Hi all. I came into histology through the "back
> door", I received excellent on-the-job training but
> never any "official" or "formal" type histology
> training as I assume one would pursue to get
> certification. (I work in a research setting, not
> clinical.)
>
> Anyway, I have one of these "tamper" devices & have
> never used it. Always wondered what it was for...
> Now that I see it is called a "tamper", I imagine it
> is for holding a piece of tissue flat onto the bottom
> of the embedding mold? (I always just use forceps...)
> If that is indeed what it is for, how do you hold the
> tamper? With forceps?
>
> Thanks in advance for helping to educate me!
>
> Miriam Schroeder
>
> __________________________________________________
> Do You Yahoo!?
> Get Yahoo! Mail - Free email you can access from anywhere!
> http://mail.yahoo.com/
>
>
> - ----------
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> with ESMTP (Fabrik F07.3-000)
> id SINN.18007151@paris.fabrik.com ; Tue, 10 Oct 2000 10:32:42
> -0800
> Received: from 129.112.18.39 ([129.112.18.39])
> by SWVX12.SWMED.EDU (PMDF V6.0-24 #46721)
> with SMTP id <01JV67K70KH09EDS9S@SWVX12.SWMED.EDU> for
> nancy.klemme@sakuraus.com; Tue, 10 Oct 2000 12:30:42 -0500 (CDT)
> Date: Tue, 10 Oct 2000 09:53:19 -0700 (PDT)
> From: Miriam Schroeder <vitalred@yahoo.com>
> Subject: How/why/when do you use a "Tamper"?
> To: histonet@pathology.swmed.edu
> Message-id: <20001010165319.71736.qmail@web9701.mail.yahoo.com>
> MIME-version: 1.0
> Content-type: text/plain; charset=us-ascii
>
> - ----------
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 14:12:50 -0500
> From: "Bryan Llewellyn" <bryand@netbistro.com>
> Subject: Re: Flooring surfaces in Histology
>
> The floor area in our lab is made of lino and is constantly getting
> slippery. There have been several near misses over the last few years,
> including the Manager of Housekeeping and the Medical Director. The most
> effective way we have found is to have the floor cleaned with an abrasive
> cleaner (Ajax) using a floor scrubber with large rotating pads. Once a
> month seems to work OK. We have to be very specific that no polish is to
> be
> applied afterwards.
>
> Bryan Llewellyn
>
> - ----- Original Message -----
> From: "Kathy Paton" <Patonk@WHL.co.nz>
> To: <histonet@pathology.swmed.edu>
> Sent: October 9, 2000 8:26 PM
> Subject: Flooring surfaces in Histology
>
>
> > Dear All
> >
> > Sorry to raise a well worn issue. Wax on the floors in the microtomy
> > area.
> > We have moved into a new laboratory with heavy duty lino with no special
> > abrasive surface.
> > Within three months we had a few very nasty stingey hurtee type falls.
> > With Health and Safety foremost in my
> > Mind I contacted a company that supplies a special type of lino with a
> > metallic inset.
> > We have also established a more intensive cleaning up process.
> > However the architects who are designing the remainder of the laboratory
> > have assured us that this is a "process problem". And that no special
> > surface should be necessary. The genius even suggested putting the
> > microtomists into "little rooms" with vacuums surrounding them!!!!!!
> > Has anybody some enlightening suggestions for this old problem or any
> > similar experiences.
> > Frank Lloyd Wright...........come in please.
> > Regards
> > Kathy Paton
> > Team Leader
> > Surgical Pathology Unit
> > North Shore Hospital
> > Auckland
> > New Zealand
> >
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 14:13:04 -0500
> From: ajennings@unmc.edu
> Subject: Re:How/why/when do you use a "Tamper"?
>
>
> Now that I heard Nancy's explanation on how to use a tamper I have to have
> one.....who was that you could get them from??? just kidding please DON"T
> resend vendors names anita
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 14:51:16 -0500
> From: Miriam Schroeder <vitalred@yahoo.com>
> Subject: Thanks for Tamper info! + Question-Large Tampers (?)
>
> Hi all. Thanks for the very helpful replies you have
> sent regarding the use of tampers. I can't believe
> one of these incredibly useful-sounding items has been
> sitting at my embedding center all this time, without
> me knowing how to use it. I can think of numerous
> instances when it would have come in really handy, had
> I used it!
>
> It sounded from earlier discussion like the tampers
> come in 10x10 mm & 5x5 mm sizes. Does anyone know of
> a tamper to fit the large (38x25) molds? This would
> be especially useful to me as I often have many small
> samples which need to be in the same cutting plane (as
> Nancy Klemme mentioned in her reply).
>
> Thanks so much!
> Miriam Schroeder
>
> __________________________________________________
> Do You Yahoo!?
> Get Yahoo! Mail - Free email you can access from anywhere!
> http://mail.yahoo.com/
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 15:24:07 -0500
> From: "Awbrey, Donald" <DonaldAwbrey@texashealth.org>
> Subject: Re: Tamper
>
>
> Histonetters,
>
> Sometimes it is worth your time to "Tamper" with your
> embedding materials........
>
> I think I should stick with my day job!!
>
>
> Donald G. Awbrey, HT(ASCP) QIHC
> Electron Microscopy / Image Analysis
> DonaldAwbrey@TexasHealth.org
> donaldawbrey@hotmail.com
> (817)-878-5647
>
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 15:50:37 -0500
> From: RSRICHMOND@aol.com
> Subject: Controls for lipofuscin stains
>
> I'd suggest as a control normal liver or brain, selected for abundant -
> what
> was once called "wear and tear pigment". The control should be fixed and
> processed the same way the material being examined is.
>
> The word is "lipofuscin" - pronounced ligh-po-FUSS-in, derived from a
> Latin
> word "fuscus" which can mean brown, yellow, or dark. The dye name
> "fuchsin" -
> FYOOK-sin - is derived from a flower called a fuscia (FYOO-sha) which was
> named after someone with the German name Fuchs (pronounced FOOX).
>
> The seaweed Fucus (whence fucose) is etymologically unrelated, as is,
> well,
> like, y'know, forget it....
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 15:50:54 -0500
> From: RSRICHMOND@aol.com
> Subject: Re: Tampers
>
> These tampers are a non-trivial item, and can be quite hard to locate,
> particularly in a laboratory - and I've been in many like this - where the
>
> histotechnologist has no access to the catalogs locked up in the lab
> manager's office.
>
> In one lab I worked in, we went from 25% to 90% of the length of a
> prostate
> biopsy actually getting on the slide, after we were able to obtain
> tampers.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
> ----------------------------------------------------------------------
>
> Date: 10 Oct 2000 15:51:26 -0500
> From: aidan.schurr@hvh.co.nz
> Subject: Re: Flooring surfaces in Histology
>
> Hi Kathy,
>
> We have just purchased some new mats, which are composed of
> 'bubbles' of rubber approx 3cm in diameter and 1.5 cm high. Not
> only are they non slip and very easy to clean (we use a vacuum
> cleaner), they are extremely soft to stand on - anti fatigue for our
> microtomists. I got them from a company called Business Office
> Interiors here in Wellington. They are 1200mm x 900mm, and cost
> about $100 each. Only drawback is that they are natural rubber,
> so don't spill any xylene on them!
>
> Cheers,
> Aidan
>
> > Dear All
> >
> > Sorry to raise a well worn issue. Wax on the floors in the microtomy
> > area.
> > We have moved into a new laboratory with heavy duty lino with no special
> > abrasive surface.
> > Within three months we had a few very nasty stingey hurtee type falls.
> > With Health and Safety foremost in my
> > Mind I contacted a company that supplies a special type of lino with a
> > metallic inset.
> > We have also established a more intensive cleaning up process.
> > However the architects who are designing the remainder of the laboratory
> > have assured us that this is a "process problem". And that no special
> > surface should be necessary. The genius even suggested putting the
> > microtomists into "little rooms" with vacuums surrounding them!!!!!!
> > Has anybody some enlightening suggestions for this old problem or any
> > similar experiences.
> > Frank Lloyd Wright...........come in please.
> > Regards
> > Kathy Paton
> > Team Leader
> > Surgical Pathology Unit
> > North Shore Hospital
> > Auckland
> > New Zealand
> >
> >
> >
>
>
> ___________________________________________________
> shin: device for finding furniture in the dark...
> ___________________________________________________
> a i d a n c s c h u r r
> section head, histology department
> hutt valley health
> high street
> lower hutt, new zealand
>
> ph. ++64 4 5709173
> fax ++64 4 5709214
> ___________________________________________________
>
>
>
> Here are the messages received yesterday!
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