Re: IHC counterstain
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From: | Amos Brooks <atbrooks@snet.net> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi,
Chloroform is nasty stuff. Have you ever tried Acetone (not that that is
all that much of an improvement in safety). The results are the same for us.
Amos Brooks
Laurence Reilly wrote:
> Hi all,
> If the chromagen was DAB then Methyl Green (cleared with chloroform)is a
> useful counterstain. It gives a pleasant green background and it also
> stains the nuclei.
>
> Regards, Laurie.
>
> Mr.Laurie Reilly Ph 07 4781 4468
> Physiology & Pharmacology Fax 07 4781 5558
> Aust.Inst.of Tropical Vet.& Animal Sc.
> James Cook University laurie.reilly@jcu.edu.au
> Townsville Qld. 4811
> Australia.
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