For information I used Mayer's Haematoxylin then alcohol, Shandon
xylene
substitute and mounted in Entellan from xylene.
Ian.


>To: ian.montgomery@bio.gla.ac.uk
>Cc: histonet@pathology.swmed.edu
>Date: Thu, 11 Nov 1999 06:12:07 EST
>Subject: Re: DAB: impermanent?
>X-Mailer: Juno 2.0.11
>From: Jeffrey S Crews <cruzetti@juno.com>
>
>Has anyone else had the situation occur in which good nickel-enhanced DAB
>(either fresh or out-of-date) vanishes or decreases after
>alcohols/clearing? We have had some runs where I would swear that this
>had happened; it looked good during the reaction, but after clearing the
>staining was significantly reduced. Just how permanent is DAB anyway, and
>under what conditions can you get it to wash out like this? I'd love to
>hear from you all on this. thanks.
>							jc
>
>On Thu, 11 Nov 1999 09:25:42 +0000 Ian Montgomery
><ian.montgomery@bio.gla.ac.uk> writes:
>>	My question yesterday regarding DAB was just to gauge reaction
>>as to old
>>DAB kits. I hadn't noticed, but my kit expired last April and until
>>recently had worked fine. Yesterday I popped through some sections in
>>the
>>normal way and checked the DAB reaction with a microscope. Beautiful
>>result, clean as a whistle and good and black (nickel enhanced).
>>After
>>counterstaining and DCM the reaction product had gone, vanished like
>>'snaw
>>aff a dyke'. From comments received I think I will just buy fresh and
>>pay
>>more attention to expiry dates.
>>Ian.
>>
>>
>
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