Re: Calretinin on cytospins revisisted
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From: | DDittus787@aol.com (by way of histonet) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
I use Zymeds Calretinin and have used it on cytospins, have you tried a harsh
microwave retrieval? based on an 800 watt microwave,set at high for 5
min,then at 30% power for 11.30 min in a citrate retrieval fluid, cool then
rinse in di h20 and incubate for 30 min at 37 degrees, we get gorgeous
staining.
Dana Dittus
Histology Supervisor
Abington Memorial Hospital
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