I know I've inquired about this subject in the past and I've tried some
things that were suggested but I seem to have misplaced the rest of the info
I had from Histonet.  In a nutshell, I'm trying to stain cytospins with
calretinin.  I've tried fixing the prepared slides in PAF (picric acid
formalin) for 3" and 10", with and without something called SedFix.  None of
these trials seemed to work.  Can anyone out there offer any further
suggestions?

Thanks in advance,

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568