Hi All,
          We've recently been having some trouble with our Placental
Alkaline Phosphatase (Plap).  i.e. variable results. Some tumours we
expected to be positive, have been negative although the control (
normal placenta) has been strongly positive. In one case recently, a
tumour in testes (well fixed), allthough the tumour cells were
unexpectedly negative, there was positive staining in smooth muscle
fibres.
	We use Dako's Primary (M7191, Clone 8A9) at 1:50 for 30mins ,
microwave/pressure cook, and use Biogenix's HRP Kit and DAB. Staining is
carried out on the Optimax II.
	Anyone else had any similar problems with this ??????
		Regards
			Alex. Brown
			Crosshouse Hospital
			Kilmarnock, Scotland.