[Histonet] Fixing question
dear folks, pardon my ignorance but....
It seems that pressure is applied to the processing of tissues to
ensure evenness and to force out any residual alcohol and/or xylene
that might over-dry one's delicate samples.
I gather that depending on what machine you have you can apply pressure
at each step or just at the final paraffin stage ( as in my case).
Here is my question... would applying pressure during the fixation of
the tissues also be beneficial?
Would it speed up the process therefore minimizing the possibility of
over-fixation?
Do any of you fix tissue under pressure and, if so, how do you go about
it? Just curious....
Cheers
Chris
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