RE: [Histonet] Re: tape
In all
fairness I feel that I have to add my $.02 worth. In my last research
position (a number of years ago) I needed to section cross sections of
frozen rabbit heart that had had radioactive elements perfused through it.
We needed adjacent sections that would be as identical as possible in order
to overlay autoradiographic images and histochemically
stained images. Using the Instrumedics tape transfer system, I
was able to produce exquisite, near identical sections that enabled us
to compile the data we needed for our research project. Granted, I
never tried the system with paraffin sectioning but at least in sectioning
frozens, the system worked brilliantly for me.
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital
& Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland
Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
Thank you for your input. Not a lot of people want to talk about the
problems with the Tape-Transfer System. We also have two in our lab and
I have tried on several occasions to work with Instrumedics to understand
how it can be used. Eventually they stopped emailing me which ended our
collaboration. Now I don't recommend the tape method to anyone. I
have published two articles on Array Instruction in The Journal of
Histotechnology and I thought at one time that the Tape Method could me a good
alternative for techs that have a problem cutting arrays. But now
I tell them to find someone in their area that can cut well.
Thanks again,
Thom Jensen
For more information on Tissue Microarray Instruction visit: www.arrayworkshop.com
>From: "Murphy, Linda M."
>To: "'tissuearray@hotmail.com'"
>Subject: tape
>Date: Thu, 6 Nov 2003 13:29:53 -0600
>
>Tom,
>I am so excited that people are publicly voicing just how they
feel about this most horrendous tape method. First off, I have felt all along
that it is an insult to any HistoTech who cuts........the two technicians who
do the cutting in my lab cut absolutely gorgeous sections.......special
attention is paid to the TMA's and they get consistent beautiful sections with
little to no distortions. My lab director was so impressed, however, by the
articles she had read about the "success" of the tape method, we were asked to
buy one for each cutting station.....they now just sit in the drawer in case a
researcher feels the need. I have a feeling we will end up tossing them in due
time. I am all for the poll. I no longer participate in the Histonet and was
given this information from a friend. You are certainly more than welcome to
express my views if you like. I would though like to state this is nothing
against Bernice and Instrumedics. They have known for quite some time that
their tape method needs work. I would hope they would benefit from the
information they would receive from a poll such as you would like to
undertake. Please feel free to contact me at any time.......thanks. Linda
>
>Linda M. Murphy, HT(ASCP)
>Supervisor, TACMA Shared Resource
>10-36 Guggenheim
>Mayo Foundation
>Rochester, MN 55905
>Voice:(507)266-5115
>Fax:(507)284-8105
>email:murphy.linda@mayo.edu
>
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