Dear all,
I am a freshly starting PhD student and my project concerns mainly dealing
with mouse brain frozen sections. My first work was just to practice
histology was to cut 40 microns coronal sections and nissl stain them.
Everything worked fine except that the sections started to fold when
stained (although I mounted them on a gelatine coated slides and used
gelatine to fix them more on the slides).
PS: the mouse from which the brain was taken was perfused with this procedure:
-PBS
-sulfid
-parafolmaldehyde+picric acid
and then the brain was placed in 30%sucrose for long time until used.

I appreciate it if you have any suggestions before I proceed with my work.

Best wishes,
nada





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