We've done up to P6 mice.

We use a "syringe pushing" pump. Use two syringes (one  with PBS and one
with PFA) hooked up to thin tubing and a two way valve, more tubing then an
adapter  that fits on a 30 gage needle, with the needle part fitting into
the thin catheter (this is hard to explain), we added a thin flexible
needle (but it's not sharp, sorry I don't know where we got it or what they
are used for, I'll try and find out more)  onto the end of the catheter.
We pierce the ventricle with the needle and hold it there , cut the right
atrium  wash for 30" with PBS then 5 ' of PFA 4% ( flow rate of
0.25ml/min). If you have a hard time seeing what your doing use a
magnifying glass like some people use for a microtome, or a dissecting
microscope.

hope this helps...good luck,

Nancy Walker
Molecular Biology Scientist

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker@sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001





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