Histonetters-

Has anyone out there tried the Zinc Acetate/Zinc chloride fixation method
for fixation-sensitive antigens? This fixation method is followed by
paraffin processing. Also, has anyone tried the AmeX (acetone-methyl
benzoate-xylene) tissue processing method?

I am interested in alternative fixation/processing schedules for sensitive
antigens. Antigen retrieval methods are not an option and frozen sections,
while acceptable, do not always provide excellent morphology.

Thanks for the advice!


Lorraine Gibbs
Physiology &Biophysics
University of Washington
Seattle, WA