Gayle,

Thanks for the kind words, but I don't deserve the credit for following
instructions. I have and always will advocate the One Step Trichrome as
outlined in the AFIP 3rd edition. I have never modified it and have find the
stain to produce consistent results. What is really nice is that by
substituting the Aniline Blue for the Light Green, you have now replaced the
Masson's Trichrome. If you are in a large facility such as I am currently, with
many pathologists, this stain will allow you to accommodate every whim.

Victor in Seattle

Gayle Callis wrote:

> Hopefully,
>
> Victor Tobias will come through on this one, he put a one step trichrome on
> Histonet some time ago.  It should be in Histonet Archives.  I remember his
> as being simple and very good.
>
> At 04:45 PM 11/17/00 -0800, you wrote:
> >Does anyone sell a one-step trichrome kit, or just the trichrome
> >itself? It's a long time since I did any proper histology, but now I have
> >to do a trichrome a few times on a few slides. It doesn't seem worth
> >buying and making up all the different stains, and I think I can get by
> >without the Bouin's step. So, if anyone knows where I can buy the stuff
> >please tell me. Alternatively, as a long shot, if anyone at UBC happens to
> >read this and happens to have a little stain to spare....
> >       Thanks.
> >
> >Lesley Weston.
> >
> >
> >
> >
> >
> >
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
> 406 994-4705
> 406 994-4303