-----Original Message-----
From: Jim Ball <xryhisto@ovis.net>
To: Histonet@pathology.swmed.com <Histonet@pathology.swmed.com>
Date: Wednesday, May 19, 1999 8:10 PM
Subject: histofreeze


>Has there ever been any research done on how histo-freeze affects IHC
>prcedures, when it is sprayed directly on the face of a tissue block
>(embeded in wax) in order to obtain a section. I personally detest the
>stuff, but everyone in the deparment swears by the product. The only
>evidence That I have that it might be effecting the IHC procedures is that
>my S100 and HMB45 control block seemed to lose staining intensity the more
>times it was exposed to the spray. There is no way the powers to be will
>listen to any thing I say unless it comes from outside their little
>universe. My main concern is that weak reactive sites may be lost
completely
>by even short exposures to histo-freeze.
>