DAB Chromagen -Reply
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From: | "Richard Cartun" <Rcartun@harthosp.org> |
To: | <AEJNSN@aol.com>, <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Tue, 25 May 1999 20:54:03 -0400 |
Content-Type: | text/plain; charset=US-ASCII |
I have always been a strong proponent of AEC (high contrasting color); however, we have switched most of our immunoperoxidase stains to DAB because of convenience. We use DAB+ from DAKO and have been very pleased. It is very easy to prepare (1 mL of substrate buffer + 1 drop of liquid DAB+ solution). We apply the DAB+ to our tissue sections for 10 minutes. It gives strong, uniform, and consistent staining. I still use AEC+ (also from DAKO) with certain infectious disease antibodies (where I need differentiate immunoreactivity from endogenous pigments), as well as with melanocytic lesions/tumors.
R. Cartun
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