You can fix the tissue overnight either cold or at room temp. but osmium 
will only penetrate about 1mm no matter how long you fix. And it will 
make the tissue
VERY brittle as Phil mentioned. Seriously consider cutting frozen 
sections and osmicating them, no penetration or brittle sections problems!
You MUST open the bottles/vials etc under a hood, osmium will fix your 
corneas!

Geoff

Nuernberger, Barb wrote:
> I'm asking this on behalf of my histologist who has been asked by a
> pathologist to modify a protocol that she has from the AFIP manual.
> When doing an osmium fixation on formalin fixed tissue, is the 8 hour
> fixing time a minimum or can it sit overnight before continuing?
>
> Thank you for any help.
> Barb Nuernberger
> Chief Toxicologist
>
>
> Medical Examiner's Office
> 2100 Jefferson St. 
> Jacksonville,FL 32206
> (904)630-5470
> BNuern@coj.net
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



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