I think I read, while studying for the IHC exam, that the reason the acetone is cold is to keep the tissue from washing off.  It may not be necessary now because of the good quality charged slides available.

-------------- Original message -------------- 
From: "Charles, Roger"  

> Does any know or remember why acetone, when used as a fixative, is 
> always used "ice cold" or in our case at -20C? 
> 
> 
> 
> 
> 
> Roger Charles 
> 
> Microbiologist 
> 
> Pennsylvania Veterinary Laboratory 
> 
> 2305 N Cameron St 
> 
> Harrisburg, PA 17110 
> 
> 717-787-8808 
> 
> 
> 
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