Does anyone have pricing for private autopsy, complete, partial, brain only, etc?
Annette Featherstone
Kaleida Health
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Friday, May 25, 2007 12:57
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 42, Issue 37
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: telephone solicitations (Gayle Callis)
2. Histo Tech Needed in Indianapolis (Pam Barker)
3. Tennessee Society Hotel Deadline is Today (Hofecker, Jennifer L)
4. Shirley is away 24 May 2007 afternoon ... (Shirley PHUA)
5. Job posting (pam johnson)
6. Job posting (pam johnson)
7. RE: Nikon NIS-Elements - digital camera software problem;
software others use?? (Tamara Franz-Odendaal)
8. Abcam survey (Carl Hobbs)
9. Abcam survey (Carl Hobbs)
10. Mouse specific M2/M6 antibody staining protocol (tenny jin)
11. RE: IHC cytology specimens (C.M. van der Loos)
12. Neurogenesis (Ian Montgomery)
13. DAPI staining methacrylate sections (matt prideaux (RI))
14. RE: IHC cytology specimens (Joanne Mauger)
15. Custom made solutions (Tim Wheelock)
16. RE: Custom made solutions (Carole Fields)
17. test slides for detection kits (Vickroy, Jim)
18. RE: Neurogenesis (Alan Bright)
19. Stainer Comparison (Breeden, Sara)
20. Donation of Slides? (Breeden, Sara)
21. Re: Stainer Comparison (Rene J Buesa)
22. FW: Ventana Users (Mike Pence)
23. help with some references (Liz Chlipala)
24. gordon and sweetsd retic stain (Diana McCaig)
----------------------------------------------------------------------
Message: 1
Date: Thu, 24 May 2007 11:01:32 -0600
From: Gayle Callis
Subject: Re: [Histonet] telephone solicitations
To: Geoff McAuliffe ,
Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20070524104853.01b09530@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Geoff,
Yes, and is the reason why I removed my phone and FAX numbers from signature for email postings. I have noticed some Histonetters do not put their phone numbers in signatures either. I also received unsolicited FAXs too. I tell them to not call back and not always very diplomatically.
At 09:45 AM 5/24/2007, you wrote:
>Dear Lists:
>
> I got another telephone call at my lab yesterday from a solicitor.
> My phone number here is unlisted and does not show up on any caller
> id. I am getting 1 or 2 of these calls a week, too many to be random
> hits. I think some companies are harvesting my number from Listserv
> postings. Has anyone else had this experience?
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
------------------------------
Message: 2
Date: Thu, 24 May 2007 13:30:53 -0400
From: "Pam Barker"
Subject: [Histonet] Histo Tech Needed in Indianapolis
To: "'Histonet'"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters
I am currently working with a client in Indianapolis that is in need of an ASCP certified or eligible histo tech.
This is a permanent full time dayshift position. The client offers a
great lab environment, a great crew to work with and excellent benefits and salary.
If you think you or someone you know might be interested in more detailed information about the opportunity, please contact me.
I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1@earthlink.net
------------------------------
Message: 3
Date: Thu, 24 May 2007 13:01:47 -0500
From: "Hofecker, Jennifer L"
Subject: [Histonet] Tennessee Society Hotel Deadline is Today
To:
Message-ID:
<898D946569A27444B65667A49C074052D0E251@mailbe06.mc.vanderbilt.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hello out there,
Just wanted to remind everyone that the deadline for the hotel for the TSH meeting is today. After today, unused rooms will be released and may not be available. The meeting is June 7-9 in Dickson, Tn. If you haven't registered yet, it's not too late. We can register folks for classes up to and including the date of the meeting. The full meeting program may be accessed on the NSH website www.nsh.org under the calendar for region 3. The schedule at a glance is there with a place to download the complete program. Please email me if you have any questions or if we may be of any assistance to you.
Thanks and enjoy the rest of the week,
Jennifer
Jennifer Hofecker HT(ASCP)
Vanderbilt University Medical Center
Neuropathology Laboratory
Nashville TN
phone: (615)343-0083
fax:(615)343-7089
TSH President
NSH Quality Control Committee Chair
------------------------------
Message: 4
Date: Fri, 25 May 2007 02:14:40 +0800
From: Shirley PHUA
Subject: [Histonet] Shirley is away 24 May 2007 afternoon ...
To: histonet
Message-ID:
Content-Type: text/plain; charset=US-ASCII
I will be out of the office from 24-05-2007 to 25-05-2007.
I will return on 25 May 2007.
Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg
Regrets for the inconveniences caused.
------------------------------
Message: 5
Date: Thu, 24 May 2007 14:53:51 -0400
From: pam johnson
Subject: [Histonet] Job posting
To: histonet@lists.utsouthwestern.edu
Message-ID: <8C96C4C01079F27-1920-17B3@WEBMAIL-RA14.sysops.aol.com>
Content-Type: text/plain; charset="utf-8"
Job Title: Supervisor, ARC Histology (Job Number: 15541)
Job Location: Memphis, TNÂ Â Â
Â
St. Jude Children's Research Hospital, located in Memphis, Tennessee, is a premier center for research and treatment of potentially fatal childhood diseases, including cancer and certain blood, genetic, and immunodeficiency disorders. The hospital's mission is to advance cures, and means of prevention, for pediatric catastrophic diseases through research and treatment. St. Jude is dedicated to providing unsurpassed patient care and to advancing the health of children through biomedical research.
Â
Currently, St. Jude Children's Research Hospital has an opening for Supervisor- ARC Histology in the Animal Resources Center (Job Number 15541).
Â
This position supervises and effectively manages staff of the Veterinary Histology laboratory. Supervises and performs laboratory procedures and the interpretation and reporting of results. Assists with the evaluation and testing of new procedures and/or instrumentation; establishes maintenance protocols. Ensures accurate records and quality control procedures are implemented and maintained as required by the hospital and accrediting, licensing/regulatory agencies (e.g. State of Tennessee, AAALAC, and USDA). Also coordinates the laboratory budget.
Â
The successful candidate will have the following qualifications:
A High school diploma or GED plus six (6) years of experience as a Histotechnologist or Histotechnician
A Bachelor’s degree or equivalent (in concordance with current Federal and State Regulations) is preferred
Veterinary histology experience is preferred
Supervisory experience is also preferred
Certification as a Histotechnologist or Histotechnician by the American Society of Clinical Pathology Board of Registry is required.Â
Subspecialty qualification in a related area (e.g. Immunohistochemistry) is preferred.
Â
Â
St. Jude Children's Research Hospital is located in Memphis, a city rich in history and culture, with several outstanding theaters, museums, and nature preserves. Housing and other living expenses are very reasonable.
Â
Best Place to Work in Academia chosen by the readers of The Scientist.
Â
St. Jude Children's Research Hospital offers an excellent salary and benefits package. Qualified applicants may apply via our online process at www.stjude.org/jobs, referencing Job Number 15541.
Â
www.stjude.org
An Equal Opportunity Employer
__________________________________________________________________________________________________________________________
Â
Â
Â
Â
Â
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com.
------------------------------
Message: 6
Date: Thu, 24 May 2007 14:58:32 -0400
From: pam johnson
Subject: [Histonet] Job posting
To: histonet@lists.utsouthwestern.edu
Message-ID: <8C96C4CA8D481B1-1920-17EC@WEBMAIL-RA14.sysops.aol.com>
Content-Type: text/plain; charset="utf-8"
Job Title: Supervisor, ARC Histology (Job Number: 15541)
Job Location: Memphis, TNÂ Â Â
Â
St. Jude Children's Research Hospital, located in Memphis, Tennessee, is a premier center for research and treatment of potentially fatal childhood diseases, including cancer and certain blood, genetic, and immunodeficiency disorders. The hospital's mission is to advance cures, and means of prevention, for pediatric catastrophic diseases through research and treatment. St. Jude is dedicated to providing unsurpassed patient care and to advancing the health of children through biomedical research.
Â
Currently, St. Jude Children's Research Hospital has an opening for Supervisor- ARC Histology in the Animal Resources Center (Job Number 15541).
Â
This position supervises and effectively manages staff of the Veterinary Histology laboratory. Supervises and performs laboratory procedures and the interpretation and reporting of results. Assists with the evaluation and testing of new procedures and/or instrumentation; establishes maintenance protocols. Ensures accurate records and quality control procedures are implemented and maintained as required by the hospital and accrediting, licensing/regulatory agencies (e.g. State of Tennessee, AAALAC, and USDA). Also coordinates the laboratory budget.
Â
The successful candidate will have the following qualifications:
A High school diploma or GED plus six (6) years of experience as a Histotechnologist or Histotechnician
A Bachelor’s degree or equivalent (in concordance with current Federal and State Regulations) is preferred
Veterinary histology experience is preferred
Supervisory experience is also preferred
Certification as a Histotechnologist or Histotechnician by the American Society of Clinical Pathology Board of Registry is required.Â
Subspecialty qualification in a related area (e.g. Immunohistochemistry) is preferred.
Â
Â
St. Jude Children's Research Hospital is located in Memphis, a city rich in history and culture, with several outstanding theaters, museums, and nature preserves. Housing and other living expenses are very reasonable.
Â
Best Place to Work in Academia chosen by the readers of The Scientist.
Â
St. Jude Children's Research Hospital offers an excellent salary and benefits package. Qualified applicants may apply via our online process at www.stjude.org/jobs, referencing Job Number 15541.
Â
www.stjude.org
An Equal Opportunity Employer
__________________________________________________________________________________________________________________________
Â
Â
Â
Â
Â
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com.
------------------------------
Message: 7
Date: Thu, 24 May 2007 17:35:19 -0300
From: "Tamara Franz-Odendaal"
Subject: RE: [Histonet] Nikon NIS-Elements - digital camera software
problem; software others use??
To:
Message-ID:
Content-Type: text/plain; charset=US-ASCII
HI
you may want to check the specifications of your computer. I am about to purchase hte system and have been told that some microchip data sets in some PCs conflict with the NIS elements software - they will only guarantee the software working with some PCs - i.e. some pentium 4's and not all of them. I suggest you call the imaging experts at Nikon to discuss.
Tamara
Tamara Franz-Odendaal (PhD)
Assistant Professor of Biology,
Mount Saint Vincent University
166 Bedford Highway
Halifax, NS, B3M 2J6
Canada
Tel: +1 902 - 457 6140
Fax: +1 902 - 457 6455
http://faculty.msvu.ca/tfodendaal/
>>> "Connolly, Brett M" 05/24/07 12:18 PM >>>
Gustave,
I use NIS-Elements and have not experienced any of those problems. Ours was installed earlier this year.
Brett M. Connolly, Ph.D.
Research Fellow
MRL, Imaging Research
Merck & Co., Inc.
WP-44K
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly@merck.com
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert
Sent: Thursday, May 24, 2007 11:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use??
Hello,
Is anyone out there using a Nikon camera with NIS-elements (Advanced
Research) software for digital imaging capture and digital image analysis??
Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again.
Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed.
So my questions are as follows:
Do you currently us NIS-Elements? If so, have you experienced similar issues?
What image capturing software is the BEST both in terms of analysis and image capture?
If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance.
Gustave Hebert
Scientist II
Wyeth Research
Cambridge MA
---------------------------------
8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut.
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This communication, including any attached documentation, is intended only for the person or entity to which it is addressed, and may contain confidential, personal, and/or privileged information. Any unauthorized disclosure, copying, or taking action on the contents is strictly prohibited. If you have received this message in error, please contact us immediately so we may correct our records.
Please then delete or destroy the original transmission and any subsequent reply.
Thank you.
------------------------------
Message: 8
Date: Thu, 24 May 2007 21:52:50 +0100
From: "Carl Hobbs"
Subject: [Histonet] Abcam survey
To: "Histonet"
Message-ID: <006e01c79e45$7dc8c470$4101a8c0@carlba65530bda>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
They are interested in finding out how many of us Immunogeeks need Abs specifically directed against animal proteins.
I have never encountered such interest before.
Sure , many Abs are x-reactive: however, I have spent loads of money testing Abs out, that are human-reactive but their x-species reactivity is not known. ( I work with non-human sections, mostly) Many are useless in non-human pwax tissues.
I am a researcher and have no commercial "profile" ( other than accumulating Abcam points, lol) Please visit this site http://www.immunoportal.com/index.php and add any comments.
Thanks
Carlos
------------------------------
Message: 9
Date: Thu, 24 May 2007 21:52:50 +0100
From: "Carl Hobbs"
Subject: [Histonet] Abcam survey
To: "Histonet"
Message-ID: <006f01c79e45$82c1f5f0$4101a8c0@carlba65530bda>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
They are interested in finding out how many of us Immunogeeks need Abs specifically directed against animal proteins.
I have never encountered such interest before.
Sure , many Abs are x-reactive: however, I have spent loads of money testing Abs out, that are human-reactive but their x-species reactivity is not known. ( I work with non-human sections, mostly) Many are useless in non-human pwax tissues.
I am a researcher and have no commercial "profile" ( other than accumulating Abcam points, lol) Please visit this site http://www.immunoportal.com/index.php and add any comments.
Thanks
Carlos
------------------------------
Message: 10
Date: Thu, 24 May 2007 23:18:36 +0200
From: "tenny jin"
Subject: [Histonet] Mouse specific M2/M6 antibody staining protocol
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear all,
Has anyone had some hand-on experience with mouse specific M2/M6 antibody (from Iowa Hybridoma bank) to detect mouse cells transplanted in the rat brain (cryosection)? I have trouble with the high background staining. If someone could share the staining protocol, I will appreciate it.
Thanks in advance!
Tenny
------------------------------
Message: 11
Date: Fri, 25 May 2007 10:22:32 +0200
From: "C.M. van der Loos"
Subject: [Histonet] RE: IHC cytology specimens
To: histonet@lists.utsouthwestern.edu
Cc: mary.ann.deathridge@Vanderbilt.Edu
Message-ID: <67bc0c67ba84.67ba8467bc0c@amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"
Mary Ann,
I am answering this from a research point of view, totally unaware of
Her2neu guidelines or what so ever.
We fixed cytospins in NBF (5 min, RT), washed them with tap water (5
min) and then to alcohol 70 (3 hours - overnight). Wash with tap water
and perform HIER with appropriate buffer. We use half the time at max.
temperature as for FFPE's. After cooling down, wash with tap water and
perform IHC as with FFPE tissue sections (antibody, dilution,
detection).
I am aware this procedure as against all logics but it simple
works. After all, 5 min NBF fixation cannot cause any cross linking
and alcohol fixation certainly not. Therefore, HIER would not be
necessary. But after testing we saw HIER was very essential! When I
heard about this procedure I was very sceptical it would ever
work. However, I was surprised to see how well the cells survive this
harsh procedure and nicely stay at the glass. For some particular
antigens we have even done this procedure successfully with cryostat
tissue sections from fresh frozen blocks (section stays at the glass,
morphology is fine)!
Therefore folks: a theory is good to keep in mind, but testing is
sometimes better. And, I also stopped with surprising myself about
"strange" protocols.
I hope this helps.
Have a nice weekend,
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Thu, 24 May 2007 11:16:19 -0500
From: "Deathridge, Mary Ann"
Subject: [Histonet] IHC cytology specimens
To:
Does anyone in histoland have a protocol for the prep of cytology
specimens (cytospins, smears) for IHC?
Formalin fix? how long? air dried? 95% alc fix? esp. since the new
Her2nu guidelines
Thanks in advance
Thanks
MaryAnn Deathridge, HT (ASCP)
Supervisor, Histopathology
Vanderbilt Un. Med. Ctr
Nashville, TN
------------------------------
Message: 12
Date: Fri, 25 May 2007 11:53:56 +0100
From: "Ian Montgomery"
Subject: [Histonet] Neurogenesis
To: "Histonet"
Message-ID: <001501c79eba$fe917640$4724d182@ibls.gla.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
I'm just about to start my literature search for information on a new neurogenesis project. In the first instance I'll only be looking at markers for new neurones so any hints and tips pointing me in the right direction would be welcome.
Recently a topic has covered freezing tissue in iso-pentane cooled with solid CO2. This is also of interest for another project where I'll be studying skeletal muscle from salmon. My routine technique is iso-pentane cooled using liquid nitrogen and for an earlier pilot study this was the technique used. But, these salmon specimens are taken from pens in Scottish sea lochs so technically it's a wee bit tricky for the scientists freezing the specimens. Remember, this is Scotland where wind, rain and heaving seas are normal so using liquid nitrogen presents health and safety issues. A question for those who have frozen muscle using CO2 cooled iso-pentane? Can it be done successfully and repeatedly without ice-crystal damage? Obviously I'll get the people at the fish farm to try it before the experiments start but I don't want them to waste their time as in the commercial setting, time is money.
I should also point out that Scottish salmon is like the whisky, the gift of the gods.
Ian.
Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Tel:01413398855
Extn: 8511.
------------------------------
Message: 13
Date: Fri, 25 May 2007 12:09:11 +0100
From: "matt prideaux \(RI\)"
Subject: [Histonet] DAPI staining methacrylate sections
To:
Message-ID:
<84DA9D8AC9B05F4B889E7C70238CB45104C52B7E@rie2ksrv1.ri.bbsrc.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Hi everyone,
I'm trying to find a method to examine osteocyte density in areas of new bone formation (between two calcein labels). The sections that we have are 200 micron thick sections of mouse tibia. We have a confocal microscope, which gives great images of the labels but the osteocytes are not easily seen. I have heard that DAPI would be an ideal stain for the osteocyte nuclei, but is it possible to stain methacrylate embedded sections with DAPI and if so does anyone have a method for this?
Thanks,
Matt
Matt Prideaux
Bone Biology Group
Gene Function and Development
Roslin Institute
Roslin
Midlothian
EH25 9PS
Tel: +44 131 5274 244/254
Institute Fax: +44 131 440 0434
Roslin Institute is a company limited by guarantee, registered in Scotland (registered number SC157100) and a Scottish Charity (registered number SC023592). Our registered office is at Roslin, Midlothian, EH25 9PS. VAT registration number 847380013.
The information contained in this e-mail (including any attachments) is
confidential and is intended for the use of the addressee only. The
opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute
------------------------------
Message: 14
Date: Fri, 25 May 2007 08:37:24 -0400
From: "Joanne Mauger"
Subject: [Histonet] RE: IHC cytology specimens
To: ,
Cc: mary.ann.deathridge@Vanderbilt.Edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hi,
I agree with Chris. NBF actually seems to help tissue stay on slide better. I will post fix a smear or cytospin in NBF for 15 mins. that has been previously fixed in alcohol. I do HIER as I would on FFPE.
I have found that some antibodies will not show expression unless this post NBF fixation and retrieval has been done-eg. INI-1. I have also found retrieval necessary on thin prep cytospins that had been only fixed in sacamano,and 95% etoh.
Trial and error is the key.
Jo
------------------------------
Message: 15
Date: Fri, 25 May 2007 09:36:58 -0400
From: Tim Wheelock
Subject: [Histonet] Custom made solutions
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID: <4656E67A.4040309@mclean.harvard.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone:
Does anyone know of a company who makes custom-made solutions?
I am finding it too time consuming and distracting to regularly make a cryoprotectant that has 5 ingredients and requires heat.
Thanks,
Tim Wheelock
Harvard Brain Brain
Belmont, MA
P.S.
The cryoprotectant requires:
polyvinylpyrrolidone (PVP-40)
sucrose
sodium chloride
sodium phosphate monobasic monohydrate
sodium phosphate dibasic heptahydrate
ethylene glycol
and water.
Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, PHI by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer.
------------------------------
Message: 16
Date: Fri, 25 May 2007 09:19:33 -0400
From: "Carole Fields"
Subject: RE: [Histonet] Custom made solutions
To: "Tim Wheelock"
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
I ran into the same situation and contacted Poly Scientific and they made up my solution and packaged it in prefilled containers. Also BBC will do the same....I learned after the fact. Either place is great and accommodating. Let me know if you are interested and I can give you the details.
Carole Fields, HT,ASCP
Pathology Supervisor
Lexington Medical Center
2720 Sunset Blvd.
W. Columbia, SC 29169
-----Original Message-----
From: Tim Wheelock [mailto:twheelock@mclean.harvard.edu]
Sent: Friday, May 25, 2007 9:37 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Custom made solutions
Hi Everyone:
Does anyone know of a company who makes custom-made solutions?
I am finding it too time consuming and distracting to regularly make a cryoprotectant that has 5 ingredients and requires heat.
Thanks,
Tim Wheelock
Harvard Brain Brain
Belmont, MA
P.S.
The cryoprotectant requires:
polyvinylpyrrolidone (PVP-40)
sucrose
sodium chloride
sodium phosphate monobasic monohydrate
sodium phosphate dibasic heptahydrate
ethylene glycol
and water.
Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, PHI by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer.
------------------------------
Message: 17
Date: Fri, 25 May 2007 09:14:13 -0500
From: "Vickroy, Jim"
Subject: [Histonet] test slides for detection kits
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
How long are we required to keep test slides for documenting detection kits?
Jim Vickroy
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
------------------------------
Message: 18
Date: Fri, 25 May 2007 15:29:54 +0100
From: "Alan Bright"
Subject: RE: [Histonet] Neurogenesis
To: "Ian Montgomery" , "Histonet"
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Dear Ian,
On the subject of rapid freezing, I would like to make you aware that we manufacture the Bright Clini-RF Ultra low rapid freezer which is being used as a replacement for CO2 & liquid nitrogen. At present we have one type that's rapid freezing tank operates at -80 degs C and will shortly have another model for -100 degs C. There are also options available for controlling the temperature too.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery
Sent: 25 May 2007 11:54
To: Histonet
Subject: [Histonet] Neurogenesis
I'm just about to start my literature search for information on a new neurogenesis project. In the first instance I'll only be looking at markers for new neurones so any hints and tips pointing me in the right direction would be welcome.
Recently a topic has covered freezing tissue in iso-pentane cooled with solid CO2. This is also of interest for another project where I'll be studying skeletal muscle from salmon. My routine technique is iso-pentane cooled using liquid nitrogen and for an earlier pilot study this was the technique used. But, these salmon specimens are taken from pens in Scottish sea lochs so technically it's a wee bit tricky for the scientists freezing the specimens. Remember, this is Scotland where wind, rain and heaving seas are normal so using liquid nitrogen presents health and safety issues. A question for those who have frozen muscle using CO2 cooled iso-pentane?
Can
it be done successfully and repeatedly without ice-crystal damage?
Obviously
I'll get the people at the fish farm to try it before the experiments start but I don't want them to waste their time as in the commercial setting, time is money.
I should also point out that Scottish salmon is like the whisky, the gift of the gods.
Ian.
Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Tel:01413398855
Extn: 8511.
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------------------------------
Message: 19
Date: Fri, 25 May 2007 09:09:31 -0600
From: "Breeden, Sara"
Subject: [Histonet] Stainer Comparison
To:
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B8F449E@nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
I have the opportunity to purchase an automatic stainer. I'm comparing the Leica Autostainer XL and the Sakura Tissue-Tek Prisma. If you had your 'druthers, which would you choose? Would you please reply OFF LIST with your pros and cons? Thank you!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 700
Albuquerque, NM 87106
505-841-2576
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Message: 20
Date: Fri, 25 May 2007 09:11:39 -0600
From: "Breeden, Sara"
Subject: [Histonet] Donation of Slides?
To:
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B8F449F@nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Our local community college has a very popular vet(erinary) tech program; part of their training is making blood smears/touch preps/etc.
They now wash and reuse slides several times and are in need of a donation of new slides. Is there a vendor out there that could donate a quantity of frosted, basic slides to this program? Please contact me directly if you would be willing to help out. Thanks very much!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 21
Date: Fri, 25 May 2007 08:48:43 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Stainer Comparison
To: "Breeden, Sara" ,
histonet@lists.utsouthwestern.edu
Message-ID: <574862.65239.qm@web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Sakura Tissue-Tek Prisma!
René J.
"Breeden, Sara" wrote:
I have the opportunity to purchase an automatic stainer. I'm comparing the Leica Autostainer XL and the Sakura Tissue-Tek Prisma. If you had your 'druthers, which would you choose? Would you please reply OFF LIST with your pros and cons? Thank you!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 700
Albuquerque, NM 87106
505-841-2576
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---------------------------------
Don't pick lemons.
See all the new 2007 cars at Yahoo! Autos.
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Message: 22
Date: Fri, 25 May 2007 11:08:24 -0500
From: "Mike Pence"
Subject: [Histonet] FW: Ventana Users
To:
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E8@IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
-----Original Message-----
From: Mike Pence
Sent: Friday, May 25, 2007 10:03 AM
To: histonet-bounces@lists.utsouthwestern.edu
Subject: Ventana Users
I was wondering how many of you out there are using the Ventana
Automated IHC System. I would like to build a network of like users to
have as a resource when developing protocols in my IHC lab. We
currently offer about 30 standard antibodies and I work up another 1 or
2 a month. It seems that when I call Ventana for protocol assistance
they most times head me in the wrong direction. Thanks in advance for
sharing your e-mail or thoughts with me.
Mike
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Message: 23
Date: Fri, 25 May 2007 10:35:32 -0600
From: "Liz Chlipala"
Subject: [Histonet] help with some references
To:
Message-ID: <000001c79eea$b6e3b260$0d00a8c0@domain.Premier>
Content-Type: text/plain; charset="us-ascii"
Hello all
I was wondering if there was anyone out there that had copies of two
articles that they could fax or e-mail to me.
One is from the Journal of Histotechnology from 1990, I have already
requested this through the NSH web page but was hoping I could get it
sooner. Its on species IHC cross reactivity and I think that Roberta Smith
wrote it, but all I have is the following:
Smith RA. J Histotechnology 1990;13(4): 255-69
And the other is from Applied Immunohistochemistry and Molecular Morphology
also on antibody species cross reactivity
Martin CA and Badran AF, Applied Immunohistochem 1998;6(2) :84-8
I don't have titles
Thanks in advance and to all out here have a great Memorial Day weekend!
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
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Message: 24
Date: Fri, 25 May 2007 12:58:18 -0400
From: "Diana McCaig"
Subject: [Histonet] gordon and sweetsd retic stain
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Hi
I have done this stain successfuly for many years and the procedure has
never changed. Yesterday, it failed to stain any retic and the repeat
with all reagents prepared fresh gave the same results. Has anyone got
any suggestions where I can start to investigate this?
Diana
------------------------------
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End of Histonet Digest, Vol 42, Issue 37
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