Your "strange orange glow" sounds like it could be a 
mounting media problem  - have you recently changed 
bottles or did someone leave the bottle out on the bench 
(if you are using one that should be stored cold)? I've 
seen background glow when a bottle was stored improperly 
or was too old, especially with the PPD-based anti-fade 
reagents.


***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************

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