We do ours at 5 microns, brains, CNS are at 10-15 microns

sharon osborn
DNAX, SP BioPharma
Palo Alto, CA

Subject: [Histonet] section thickness
In your  invaluable, but,  no  doubt differing opinions, what  is  the
optimal section thickness   for formalin fixed paraffin processed
tissues for  immunostaining, using  DAB  as  chromogen?, there has been
some  discussion here  whether  it  should be 4,5,6, or  7microns, many
thanks.
 Richard  Edwards
 MRC TOXICOLOGY UNIT
LEICESTER..U.K.
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