Or how about a red or purple DAB replacement?  Biocare has a couple of 
permanent chromogens that can be used for HRP.  I plan to do exactly this 
for some melanin-loaded xenografts.

Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor@abbott.com




"Elizabeth Chlipala" 
Sent by: histonet-bounces@lists.utsouthwestern.edu
06/13/2005 02:58 PM

 
        To:     "'Jane Moose'" , 

        cc: 
        Subject:        RE: [Histonet] melanin renoval


Jane

Rather than perform a melanin bleach why don't you switch to a alkaline
phosphatase detection system and use fast red as your chromagen.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jane
Moose
Sent: Monday, June 13, 2005 2:53 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] melanin renoval


Does anyone have a procedure to bleach unstained slides (from FFPE
tissue) in order to remove melanin
in order to stain with DAB for  PECAM?




Jane Moose
LIS Coordinator/Histology
Newberry County Memorial Hospital
Newberry,  SC
803-405-7129

************************************************************************
************
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law.  If the
reader
of this message is not the intended recipient, you are hereby notified
that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law.  If you have received this communication in
error, please notify me immediately.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet