Alternatively, you could try Kiernan's Chromoxane cyanine R. This solution
stains in 3 minutes and gives a H&E picture. It is an acidic aqueous
solution containing Chromoxane cyanine R (CI 43820) and ferric chloride.
Reference in his book.

Another technique is to do a Diff quik on ethanol fixed frozen sections.
This will also give a H&E like picture. 

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Lee & Peggy Wenk [mailto:lpwenk@sbcglobal.net] 
Sent: Thursday, 26 May 2005 8:53 PM
To: 'wen eng'; 'histonet'
Subject: RE: [Histonet] H & E staining on frozen sections


H&E is always a 2 step stain (Hematoxylin in one step, Eosin in another,
with lots of other solutions between).

Any chance you were doing a Giemsa? Or a Paragon - A basic fuchsin/toluidine
blue combination? These are both one step.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073 


Lee & Peggy Wenk
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wen eng
Sent: Wednesday, May 25, 2005 3:00 PM
To: histonet
Subject: [Histonet] H & E staining on frozen sections

Dear histonetters,
 
Long time ago I used a H&E stain solution on frozen sections. I remember
it's a one step staining. But I couldn't find it now. Does anybody know
where I can order it? 
 
Thanks in advance!\
 
Wen

		
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