I also use Dako's Z334 anti GFAP. For me, a 1:2000 dilution followed by a 
std Dako strepABCpx-DAB after TRIS pH10 HIER is optimum. Very good on pwax 
sections of mouse, rat and chick and .....human! However, as was rightly 
advised, conditions need to be optimised locally.
If you wish, have a look at some pics on this site, p4/5 in Immunohist 
gallery
http://www.immunoportal.com 


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