Re: [Histonet] cutting angle
Hi Alan,
Help me out here, are you saying that the knife gauge angle and clearance
angle are the same? If not, then does it really matter that the gauge markings
are accurate? Setting a 1 to 3 degree clearance angle is still a problem.
Can't very well stick my head in the cryostat chamber to view the
block/knife interface from the end. And of course there is then the problem of the
bevel angle of the blade varying between manufacturers. Also, you mentioned
"flat" specimen face but wouldn't having the block parallel to the knife edge be
important also?
I have successfully set up many cryostats, in this life and in my past lives
as both a rep and a tech. I have never been able to determine a universal
method for adjusting knife angle but welcome any advice that will make me look
brilliant to my customers.
Thanks,
Dorothy
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