There is an antibody directed against tryptase, this should be an
excellent marker for mast cells only.

Joost

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Elizabeth Chlipala
Sent: dinsdag 10 mei 2005 16:41
To: histosci@shentel.net; histonet@pathology.swmed.edu
Subject: RE: [Histonet] Giemsa Stain for Mast Cells

I would use toluidine blue.  It's a great stain for mast cells.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HSRL
Sent: Tuesday, May 10, 2005 6:46 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Giemsa Stain for Mast Cells

Netters,

 

I am looking for a Giemsa stain for Mast Cells that does not have a
pinkish hue like the Gaffney Giemsa.  We are looking to have the Mast
Cells pop out at us on low power.  Any suggestions/protocols?

 

Thanks,

 

Tom

 

Tom Galati

Laboratory Director

HSRL, Inc.- A GLP Compliant Contract Laboratory

137 South Main Street

Woodstock, Virginia  22664

(540)459-8211

Fax: (540)459-8217

tomgalati@hsrl.org

www.hsrl.org

 

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