Hello,all,
 
I am doing double-immunofluorescence. Both the hosts of my secondary antibodies are donkey, so I plan to use normal donkey serum for blocking, but I do not know how to make up this solution. 
I have ordered donkey serum from sigma company, but the specification doesnot show how to make up it. so if you have done it, I hope that you can do me a favor. 
In addition, if the hosts of my secondary antiboies are different, one is horse, the other is donkey, which solution do I use for blocking? Thank you!
 
Best wishes!
 
Guofeng
 
Giessen
 
 



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