We use DAKO's BCL6 with DAKO High pH buffer, 30 minutes in a steamer, with
no problems.

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory	
Saint Francis Hospital
Tulsa, OK
japoteete@saintfrancis.com
 
> -----Original Message-----
> From:	Browning Deb [SMTP:browning@HHSC.CA]
> Sent:	Friday, May 21, 2004 12:46 PM
> To:	'histonet@lists.utsouthwestern.edu'
> Subject:	[Histonet] BCL6, EDTA buffer
> 
> Good afternoon:  As much as I dislike EDTA buffer due to the poor
> morphology
> (especially if not properly fixed) that it renders, I have not had much
> success with BCL6 staining with any other buffers.  I would appreciate any
> information on how to improve the morphology of the tissue, the use of
> other
> buffers, or any success stories, since I just can't seem to pull the
> rabbit
> out of the hat here.  Thank you.
> 
> Debra Browning, ART
> Technical Specialist, Immunohistochemistry
> Anatomic Pathology
> Hamilton Health Sciences
> phone: (905) 527-4322  ext 46131
> e-mail:  browning@hhsc.ca
> fax: (905) 524-2681
> 
> 
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