Dear Jose:

    Overnight might be allright for carbohydrates in properly fixed 
tissue, but why??  How difficult is it to not leave the slides in water 
for long periods of time? Do you really want to risk your valuable 
specimens?
Also, long times in water may tend to loosen the sections.

Geoff

Jose Luis Palazon Fernandez wrote:

>dear all list members
>
>I would like to change my question a little. Are carbohidrate (mucopolisacharids, etc) and protein histochemistry (lisine, arginine, cisteine, cistine, etc) altered if the slide is left in water for a period of time of several hours (overnight, for example) before staining.
>
>Jos#233# Luis
>
>  
>

-- 
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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